PXD014316

PXD014316 is an original dataset announced via ProteomeXchange.

Title	Nicotiana benthamiana cell pH mitigation
Description	We assessed the off-target effects of M2 expression on the leaf proteome of Nicotiana benthamiana infiltrated with the bacterial gene vector Agrobacterium tumefaciens. A iTRAQ quantitative proteomics procedure was followed to compare the leaf proteomes of plants agroinfiltrated with either an ‘empty’ vector or an M2-encoding vector. Leaves infiltrated with the empty vector had a low soluble protein content compared to non-infiltrated control leaves, associated with increased levels of stress-related proteins but decreased levels of photosynthesis-associated proteins. M2 expression partly compromised these effects of agroinfiltration to restore soluble protein content in leaf tissue, associated with restored levels of photosynthesis-associated proteins and reduced levels of stress-related proteins in the apoplast. These data illustrate the cell-wide influence of Golgi lumen pH homeostasis on the leaf proteome of N. benthamiana responding to microbial challenge.
HostingRepository	PRIDE
AnnounceDate	2019-12-06
AnnouncementXML	Submission_2019-12-06_02:42:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philippe Jutras
SpeciesList	scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-20 02:30:16	ID requested
⏵ 1	2019-12-06 02:43:00	announced

Jutras PV, Sainsbury F, Goulet MC, Lavoie PO, Tardif R, Hamel LP, D'Aoust MA, Michaud D, to Agroinfiltration. J Proteome Res, 19(1):106-118(2020) [pubmed]

submitter keyword: Nicotiana benhtamiana, iTRAQ, pH, M2 ion channel, agroinfiltration

Dominique Michaud
contact affiliation	Centre de recherche et d’innovation sur les végétaux, Université Laval, Québec, Canada
contact email	dominique.michaud@fsaa.ulaval.ca
lab head
Philippe Jutras
contact affiliation	University of Oxford
contact email	philippejutras@librem.one
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD014316

PRIDE project URI

• PRIDE
  1. PXD014316
     1. Label: PRIDE project
     2. Name: Nicotiana benthamiana cell pH mitigation