PXD014307

PXD014307 is an original dataset announced via ProteomeXchange.

Title	UBR5 in B cell development TMT
Description	Coordination of a number of molecular mechanisms including transcription, alternative splicing, and class switch recombination are required to facilitate development, activation, and survival of B cells. Disruption of these pathways can result in malignant transformation. Recently, next generation sequencing has identified a number of novel mutations in mantle cell lymphoma (MCL) patients including the ubiquitin E3 ligase UBR5. Approximately 18% of MCL patients were found to have mutations in UBR5 with the majority of mutations within the HECT domain of the protein which can accept and transfer ubiquitin molecules to the substrate. Determining if UBR5 controls the maturation of B cells is important to fully understand malignant transformation to MCL. To elucidate the role of UBR5 in B cell maturation and activation we generated a conditional mutant disrupting UBR5’s C-terminal HECT domain. Loss of the UBR5 HECT domain leads to a block in maturation of B cells in the spleen and up-regulation of proteins associated with mRNA splicing via the spliceosome. Our studies reveal a novel role of UBR5 in B cell maturation by regulating alternative splicing of key transcripts during B cell development and suggests UBR5 mutations may promote mantle cell lymphoma initiation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:50:17.647.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Samantha Swenson
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; ubiquitination signature dipeptidyl lysine; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-20 02:00:48	ID requested
1	2020-11-19 06:37:30	announced
2	2020-12-01 03:55:09	announced	2020-12-01: Updated publication reference for PubMed record(s): 32325489. 2020-12-01: Updated publication reference for DOI(s): 10.1182/blood.2019002102.
⏵ 3	2023-11-14 08:50:18	announced	2023-11-14: Updated project metadata.

Swenson SA, Gilbreath TJ, Vahle H, Hynes-Smith RW, Graham JH, Law HC, Amador C, Woods NT, Green MR, Buckley SM, UBR5 HECT domain mutations identified in mantle cell lymphoma control maturation of B cells. Blood, 136(3):299-312(2020) [pubmed]

submitter keyword: TMT UBR5 Bcells Spleen Mouse

Shannon Mychel Buckley
contact affiliation	Department of Genetics, Cell Bioliogy, & Anatomy Buckley Lab University of Nebraska Medical Center Omaha, Nebraska United States of America
contact email	shannon.buckley@unmc.edu
lab head
Samantha Swenson
contact affiliation	University of Nebraska Medical Center
contact email	samantha.swenson@unmc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD014307

PRIDE project URI

• PRIDE
  1. PXD014307
     1. Label: PRIDE project
     2. Name: UBR5 in B cell development TMT