PXD014298

PXD014298 is an original dataset announced via ProteomeXchange.

Title	The C-terminal domains SnRK2-box and ABA-box have a role in sugarcane SnRK2s auto-activation and activity
Description	Resistance to drought stress is fundamental to plant survival and development. Abscisic acid (ABA) is one of the major hormones involved in different types of abiotic and biotic stress responses. ABA intracellular signaling has been extensively explored in Arabidopsis thaliana and occurs via a phosphorylation cascade mediated by three related protein kinases, denominated SnRK2s (SNF1-related protein kinases). However, the role of ABA signaling and the biochemistry of SnRK2 in crop plants remains underexplored. Considering the importance of the ABA hormone in abiotic stress tolerance, here we investigated the regulatory mechanism of sugarcane SnRK2s - known as SAPKs (Stress/ABA-activated Protein Kinases). The crystal structure of ScSAPK10 revealed the characteristic SnRK2 family architecture, in which the regulatory SnRK2-box interacts with the kinase domain αC helix. To study sugarcane SnRK2 regulation, we produced a series of mutants for the protein regulatory domains SnRK2-box and ABA-box. Surprisingly, mutations in the SnRK2-box did not drastically affect sugarcane SnRK2 activity, in contrast to previous observations for the homologous proteins in Arabidopsis. Also, we found that the ABA-box might have a role in SnRK2 activation in the absence of PP2C phosphatase. Taken together, our results demonstrate that both C-terminal domains of sugarcane SnRK2 proteins play a role in protein activation and activity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:55:01.092.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dev Sriranganadane
SpeciesList	scientific name: Saccharum hybrid cultivar x Sorghum bicolor; NCBI TaxID: 1325291;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Xevo G2-S QTof

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-19 02:31:04	ID requested
1	2019-11-12 19:11:59	announced
⏵ 2	2024-10-22 04:55:03	announced	2024-10-22: Updated project metadata.

10.3389/fpls.2019.01105;

Righetto GL, Sriranganadane D, Halabelian L, Chiodi CG, Elkins JM, Massirer KB, Gileadi O, Menossi M, Cou, ñ, ago RM, The C-Terminal Domains SnRK2 Box and ABA Box Have a Role in Sugarcane SnRK2s Auto-Activation and Activity. Front Plant Sci, 10():1105(2019) [pubmed]

submitter keyword: SnRK2, abiotic stress, Crop plant,Abscisic Acid, sugarcane, kinase regulation

Rafael Miguez Couñago
contact affiliation	Structural Genomics Consortium, Departamento de Genética e Evolução, Instituto de Biologia,Centro de Química Medicinal (CQMED), Centro de Biologia Molecular e Engenharia Genética (CBMEG), Universidade Estadual de Campinas (UNICAMP), Campinas, SP, 13083-875, Brazil.
contact email	rafaelcounago@gmail.com
lab head
Dev Sriranganadane
contact affiliation	SGC/Unicamp
contact email	dev.sriranga@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD014298

PRIDE project URI

• PRIDE
  1. PXD014298
     1. Label: PRIDE project
     2. Name: The C-terminal domains SnRK2-box and ABA-box have a role in sugarcane SnRK2s auto-activation and activity