PXD014292
PXD014292 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Single organelle function and organization as estimated from Arabidopsis mitochondrial proteomics |
Description | Aiming at defining the protein content and composition of a single mitochondrion of Arabidopsis thaliana, shotgun analyses were performed on purified mitochondria from cultured heterotrophic Arabidopsis cells. Based on microscopic observations on the size of mitochondria, as well as biochemical properties, such as protein concentration, average molecular mass of proteins, and the average length of the signal peptide, intensity-based absolute quantification (iBAQ) values were applied to calculate the copy number of each identified protein species within a single mitochondrion. Copy numbers of the individual proteins span five orders of magnitude, ranging from >40,000 for Voltage-Dependent Anion Channel 1 (VDAC1) to sub-stoichiometric copy numbers, i.e. less than a single copy per single mitochondrion, for several pentatricopeptide repeat (PPR) proteins that modify mitochondrial transcripts. Aiming at defining the protein content and composition of a single mitochondrion of Arabidopsis thaliana, shotgun analyses were performed on purified mitochondria from cultured heterotrophic Arabidopsis cells. Based on microscopic observations on the size of mitochondria, as well as biochemical properties, such as protein concentration, average molecular mass of proteins, and the average length of the signal peptide, intensity-based absolute quantification (iBAQ) values were applied to calculate the copy number of each identified protein species within a single mitochondrion. Copy numbers of the individual proteins span five orders of magnitude, ranging from >40,000 for Voltage-Dependent Anion Channel 1 (VDAC1) to sub-stoichiometric copy numbers, i.e. less than a single copy per single mitochondrion, for several pentatricopeptide repeat (PPR) proteins that modify mitochondrial transcripts. Aiming at defining the protein content and composition of a single mitochondrion of Arabidopsis thaliana, shotgun analyses were performed on purified mitochondria from cultured heterotrophic Arabidopsis cells. Based on microscopic observations on the size of mitochondria, as well as biochemical properties, such as protein concentration, average molecular mass of proteins, and the average length of the signal peptide, intensity-based absolute quantification (iBAQ) values were applied to calculate the copy number of each identified protein species within a single mitochondrion. Copy numbers of the individual proteins span five orders of magnitude, ranging from >40,000 for Voltage-Dependent Anion Channel 1 (VDAC1) to sub-stoichiometric copy numbers, i.e. less than a single copy per single mitochondrion, for several pentatricopeptide repeat (PPR) proteins that modify mitochondrial transcripts. |
HostingRepository | PRIDE |
AnnounceDate | 2019-11-27 |
AnnouncementXML | Submission_2019-11-27_05:59:34.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Holger Eubel |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetic acid derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-06-18 07:13:45 | ID requested | |
1 | 2019-09-11 10:34:45 | announced | |
2 | 2019-10-25 05:10:04 | announced | 2019-10-25: Updated publication reference for PubMed record(s): 31520498. |
⏵ 3 | 2019-11-27 05:59:35 | announced | 2019-11-27: Updated project metadata. |
Publication List
Fuchs P, Rugen N, Carrie C, Els, ä, sser M, Finkemeier I, Giese J, Hildebrandt TM, K, ü, hn K, Maurino VG, Ruberti C, Schallenberg-R, ü, dinger M, Steinbeck J, Braun HP, Eubel H, Meyer EH, M, ü, ller-Sch, ü, ssele SJ, Schwarzl, ä, nder M, Single organelle function and organization as estimated from Arabidopsis mitochondrial proteomics. Plant J, 101(2):420-441(2020) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Plant mitochondria |
Contact List
Hans-Peter Braun | |
---|---|
contact affiliation | Leibniz University Hannover Institute of Plant Genetics |
contact email | braun@genetik.uni-hannover.de |
lab head | |
Holger Eubel | |
contact affiliation | Leibniz Universt??t Hannover |
contact email | heubel@genetik.uni-hannover.de |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD014292 |
PRIDE project URI |
Repository Record List
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