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PXD014288

PXD014288 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSensitivity and protein digestion course of proteomic Filter Aided Sample Preparation
Description1. Sensitivity of FASP was tested using SDS lysates from HeLa cells and mouse brain. Peptides were analyzed using a QExactive HF-X instrument. We found that FASP allows effective processing samples at the low and sub-microgram range. 2. Whole cell lysates of Hela cells were processed with FASP using single or double, consecutive or successive, digestion with LysC or trypsin. The generated peptides were analyzed using a LTQ-Orbitrap mass spectrometer. The results show that short digestion times apparently do not affect the number of identified proteins.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:56:50.007.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJacek Wisniewski
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-06-18 07:11:33ID requested
12019-11-11 18:12:32announced
22024-10-22 04:56:50announced2024-10-22: Updated project metadata.
Publication List
Wi, ś, niewski JR, Zettl K, Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation. Data Brief, 26():104530(2019) [pubmed]
10.1016/j.dib.2019.104530;
Keyword List
submitter keyword: FASP, Sample preparation
Contact List
Jacek R Wisniewski
contact affiliationMax Planck Institute of Biochemistry
contact emailjwisniew@biochem.mpg.de
lab head
Jacek Wisniewski
contact affiliationProteomics
contact emailjwisniew@biochem.mpg.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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