Hexaploid wheat is the most important cereal crop with the biggest planting area in the world, while its production is dramatically decreased by salt stress. Ethylene is a stress hormone, which improves salt tolerance in plants by regulating adaptive changes in gene expression level. Proteomic analysis provides an efficient method to excavate downstream functional genes in a large scale. In order to clarify the ethylene regulated salt response pathway in wheat, the roots and shoots 2-week-old wheat seedlings of cultivar ‘Qingmai 6’ treated with salt, salt and ethylene precursor ACC, and salt and ethylene signaling inhibitor 1-MCP were collected, respectively, and analyzed with untreated samples by proteomic analysis in this research.