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PXD014264 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTargeted MRM assay Complement System in Health and Infectious Disease
DescriptionThe complement system is a central component of the innate immune system. Constitutive biosynthesis of complement proteins is essential for homeostasis. Dysregulation as a consequence of genetic or environmental cues can lead to inflammatory syndromes or increased susceptibility to infection. However, very little is known about steady state levels in children or its kinetics during infection. With a new multiplex mass spectrometry-based method we analyzed 32 complement protein levels in healthy individuals and in a group of pediatric patients infected with bacterial or viral pathogens. In plasma from young infants we found reduced levels of C4BP, ficolin-3, factor B, classical pathway components C1QA, C1QB, C1QC, C1R, and terminal pathway components C5, C8, C9, as compared to healthy adults; whereas the majority of complement regulating (inhibitory) proteins reach adult levels at very young age. Both viral and bacterial infections in children generally lead to a slight overall increase in complement levels, with some exceptions. The kinetics of complement levels during invasive bacterial infections only showed minor changes, except for a significant increase and decrease of CRP and clusterin, respectively. The combination of lower levels of activating and higher levels of regulating complement proteins would potentially raise the threshold of activation, which might lead to suppressed complement activation in the first phase of life. There is hardly any measurable complement consumption during bacterial or viral infection. Altogether, expression of the complement proteins appears surprisingly stable, which suggests that the system is continuously replenished.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterEsther Willems
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListCarbamidomethyl; Oxidation; Label:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentXevo TQ-S
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-06-16 16:04:47ID requested
12019-08-06 11:00:09announced
Publication List
Willems E, Alkema W, Keizer-Garritsen J, Suppers A, van der Flier M, Philipsen RHLA, van den Heuvel LP, Volokhina E, van der Molen RG, Herberg JA, Levin M, Wright VJ, Ahout IML, Ferwerda G, Emonts M, Boeddha NP, Rivero-Calle I, Torres FM, Wessels HJCT, de Groot R, van Gool AJ, Gloerich J, de Jonge MI, Biosynthetic homeostasis and resilience of the complement system in health and infectious disease. EBioMedicine, 45():303-313(2019) [pubmed]
Keyword List
submitter keyword: targeted mass spectrometry, multiple reaction monitoring (MRM), complement system, infectious disease, C-reactive protein (CRP), clusterin
Contact List
Jolein Gloerich
contact affiliationTranslational Metabolic Laboratory, Department of Laboratory Medicine, Radboud Institute for Molecular Life Sciences, Radboud university medical center, Nijmegen, The Netherlands
contact emailjolein.gloerich@radboudumc.nl
lab head
Esther Willems
contact affiliationSection Pediatric Infectious Diseases, Laboratory of Medical Immunology, Radboud Institute for Molecular Life Sciences, Radboud university medical center, Nijmegen, The Netherlands
contact emailesther.willems1@radboudumc.nl
dataset submitter
Full Dataset Link List
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