PXD014257

PXD014257 is an original dataset announced via ProteomeXchange.

Title	Microtubules methylation LC-MSMS
Description	Microtubules are critical for mitosis, cell motility, and protein and organelle transport, and are a validated target for anticancer drugs. However, tubulin regulation and recruitment in these cellular processes is less understood. Post-translational modifications of tubulin are proposed to regulate microtubule functions and dynamics. Although many such modifications have been investigated, tubulin methylations and enzymes responsible for methylation have only recently begun to be described. Here we report that N-lysine methyl transferase KMT5A (SET8/PR‑Set7), which methylates histone H4K20, also methylates α‑tubulin. Furthermore, the transcription factor LSF binds both tubulin and SET8, and enhances α-tubulin methylation in vitro, countered by FQI1, a specific small molecule inhibitor of LSF. Thus, the three SET8, LSF, and tubulin, all essential for mitotic progression, interact with each other. Overall, these results point to dual functions for both SET8 and LSF not only in chromatin regulation, but also for cytoskeletal modification.
HostingRepository	PRIDE
AnnounceDate	2020-02-28
AnnouncementXML	Submission_2020-02-28_01:42:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	HANG GYEONG CHIN
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-14 01:30:27	ID requested
⏵ 1	2020-02-28 01:42:57	announced

Dataset with its publication pending

submitter keyword: Monomethylation, Tubulin, Human

Sriharsa Pradhan
contact affiliation	New England Biolabs, Inc
contact email	pradhan@neb.com
lab head
HANG GYEONG CHIN
contact affiliation	New England Biolabs, Inc
contact email	chinh@neb.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014257
     1. Label: PRIDE project
     2. Name: Microtubules methylation LC-MSMS