PXD014256

PXD014256 is an original dataset announced via ProteomeXchange.

Title	Embryonic day 15 chick utricle single cell analysis
Description	Single-cell proteomics can reveal the changing protein composition of differentiating cells. We used shotgun mass spectrometry to determine the abundant proteins present in single or small pools of subpicoliter-sized cells from the embryonic day 15 (E15) utricle of the chicken inner ear, when many hair cells are differentiating from progenitor (supporting) cells. The actin monomer binding protein thymosin β4 (TMSB4X) was present in E15 progenitor cells at nearly equimolar levels relative to actin, but dropped to one-tenth that value in hair cells, with little change in total actin. Single-cell RNA-seq analysis of E15 utricle cells showed that TMSB4X transcripts fell in abundance once hair-cell differentiation initiated. These results suggest that most actin is sequestered in progenitor cells, but upon differentiation to hair cells, actin is released, permitting assembly of the sensory hair bundle.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2020-01-14_06:22:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Barr-Gillespie
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-14 01:30:08	ID requested
1	2019-11-11 23:47:54	announced
⏵ 2	2020-01-14 06:22:46	announced	2020-01-14: Updated publication reference for PubMed record(s): 31682227.

Zhu Y, Scheibinger M, Ellwanger DC, Krey JF, Choi D, Kelly RT, Heller S, Barr-Gillespie PG, Single-cell proteomics reveals changes in expression during hair-cell development. Elife, 8():(2019) [pubmed]

submitter keyword: Ear, utricle, single cell, actin, thymosin beta, hair cell, supporting cell

Peter Barr-Gillespie
contact affiliation	Oregon Hearing Research Center Oregon Health & Science University Portland OR 97239 USA
contact email	gillespp@ohsu.edu
lab head
Peter Barr-Gillespie
contact affiliation	Oregon Hearing Research Center
contact email	gillespp@ohsu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD014256

PRIDE project URI

• PRIDE
  1. PXD014256
     1. Label: PRIDE project
     2. Name: Embryonic day 15 chick utricle single cell analysis