PXD014254

PXD014254 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	SLC15A4 (PHT1) interaction proteomics
Description	Human genetics and mouse models provide compelling evidence for the involvement of SLC15A4 in endosomal TLR activation. To gain an understanding of the molecular mechanism, we set out to identify its binding partners by interaction proteomics. TAP-MS/MS analysis of different SLC15A4 protein variants provided a global view of the SLC15A4 interaction landscape. Among the proteins detected, the uncharacterized protein CXorf21 emerged as a prominent and specific binder. CXorf21 is a X chromosome-encoded gene which has also been linked to SLE by a GWAS. CXorf21 was robustly identified with all the SLC15A4 constructs tested, indicating that the interaction does not require the N-terminal or central cytoplasmic region of the transporter. Reciprocal TAP-MS/MS analysis using tagged CXorf21 identified endogenous SLC15A4 as a specific interactor.
HostingRepository	PRIDE
AnnounceDate	2020-05-14
AnnouncementXML	Submission_2020-05-25_22:12:59.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014254
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ulrich Goldmann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos; Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-13 23:43:28	ID requested
1	2020-05-14 03:49:32	announced
⏵ 2	2020-05-25 22:13:00	announced	2020-05-26: Updated publication reference for PubMed record(s): 32433612.

Publication List

Heinz LX, Lee J, Kapoor U, Kartnig F, Sedlyarov V, Papakostas K, C, é, sar-Razquin A, Essletzbichler P, Goldmann U, Stefanovic A, Bigenzahn JW, Scorzoni S, Pizzagalli MD, Bensimon A, M, ü, ller AC, King FJ, Li J, Girardi E, Mbow ML, Whitehurst CE, Rebsamen M, Superti-Furga G, TASL is the SLC15A4-associated adaptor for IRF5 activation by TLR7-9. Nature, 581(7808):316-322(2020) [pubmed]

Heinz LX, Lee J, Kapoor U, Kartnig F, Sedlyarov V, Papakostas K, C, é, sar-Razquin A, Essletzbichler P, Goldmann U, Stefanovic A, Bigenzahn JW, Scorzoni S, Pizzagalli MD, Bensimon A, M, ü, ller AC, King FJ, Li J, Girardi E, Mbow ML, Whitehurst CE, Rebsamen M, Superti-Furga G, TASL is the SLC15A4-associated adaptor for IRF5 activation by TLR7-9. Nature, 581(7808):316-322(2020) [pubmed]

Keyword List

curator keyword: Biological
submitter keyword: Human, SLC, TAP, LC-MS/MS, CXorf21

curator keyword: Biological

submitter keyword: Human, SLC, TAP, LC-MS/MS, CXorf21

Contact List

Giulio Superti-Furga
contact affiliation	CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, 1090 Vienna, Austria
contact email	GSuperti@cemm.oeaw.ac.at
lab head
Ulrich Goldmann
contact affiliation	CeMM - Research Center for Molecular Medicine of the Austrian Academy of Sciences
contact email	ugoldmann@cemm.at
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD014254
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD014254

PRIDE project URI

Repository Record List

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