PXD014243

PXD014243 is an original dataset announced via ProteomeXchange.

Title	Itch Ubiquitin Ligase Deficiency and B cell LC-MSMS
Description	The E3 ubiquitin ligase Itch regulates antibody levels and prevents autoimmune disease in humans and mice, yet how Itch regulates B cell fate or function is unknown. We now show that Itch directly limits B cell activity. Using LC-MSMS, we profiled and compared the whole cell proteomes of Itch deficient and Itch sufficient CpG-stimulated B cells. B cells lacking Itch exhibited increased levels of mTORC1 targets and E2F transcription factor targets. These results support a novel role for Itch in limiting B cell metabolism and proliferation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:54:48.091.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hossein Fazelinia
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	ubiquitination signature dipeptidyl lysine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-13 01:24:25	ID requested
1	2019-07-12 04:15:22	announced
⏵ 2	2024-10-22 04:54:48	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Itch Ubiquitin Ligase, LC-MSMS, TLR9, B cell

Steven Seeholzer
contact affiliation	Proteomics Core Facility, Children's Hospital of Philadelphia, PA, USA
contact email	seeholzer@email.chop.edu
lab head
Hossein Fazelinia
contact affiliation	Children's Hospital of Philadelphia
contact email	fazeliniah@email.chop.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD014243

PRIDE project URI

• PRIDE
  1. PXD014243
     1. Label: PRIDE project
     2. Name: Itch Ubiquitin Ligase Deficiency and B cell LC-MSMS