PXD014238

PXD014238 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteome analysis of the Arabidopsis thaliana N-degron pathway E3 ligase mutant, prt1-1.
Description	The N-degron pathway relates the half-life of a protein to its amino terminal (Nt) residue. Following cleavage of proteins by endopeptidases, new N-t amino acids are revealed and can be recognised by different E3 ligase enzymes that target the substrate proteins for degradation via the proteasome. Studies with artificial proteins have shown that PRT1 is an Arabidopsis thaliana E3 ligase with specificity for aromatic N-termini (phenylalanine, tyrosine, tryptophan), but very little is known about its physiological roles and endogenous substrates. To gain insight into the impact of PRT1 on the proteome, we conducted a quantitative proteomic analysis, comparing leaf proteins from the prt1-1 loss of function mutant with those from wild type plants.
HostingRepository	PRIDE
AnnounceDate	2020-01-09
AnnouncementXML	Submission_2020-01-09_06:42:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hongtao Zhang
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-12 08:56:34	ID requested
⏵ 1	2020-01-09 06:42:32	announced

Till CJ, Vicente J, Zhang H, Oszvald M, Deery MJ, Pastor V, Lilley KS, Ray RV, Theodoulou FL, Holdsworth MJ, N-recognin E3 ligase PROTEOLYSIS1 influences the immune response. Plant Direct, 3(12):e00194(2019) [pubmed]

submitter keyword: Arabidopsis, N-degron pathway PRT1 leaves TMT- labelling

Hongtao Zhang
contact affiliation	Department of Plant Sciences Rothamsted Research, West Common, Harpenden, Herts, AL5 2JQ, UK
contact email	hongtao.zhang@rothamsted.ac.uk
lab head
Hongtao Zhang
contact affiliation	Plant Sciences Department, Rothamsted Research, Harpenden, AL5 2JQ, UK
contact email	hongtao.zhang@rothamsted.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD014238

PRIDE project URI

• PRIDE
  1. PXD014238
     1. Label: PRIDE project
     2. Name: Quantitative proteome analysis of the Arabidopsis thaliana N-degron pathway E3 ligase mutant, prt1-1.