PXD014236

PXD014236 is an original dataset announced via ProteomeXchange.

Title	Rpn4- and Proteasome- Mediated Yeast Response to Ethanol Stress: a View from a Proteome
Description	Distilled spirits production using S. cerevisiae requires understanding the mechanisms of yeast cell response to the alcohol stress. Reportedly, specific mutations in genes of the ubiquitin-proteasome system, e.g. RPN4, may result in strains exhibiting either hyper-resistance or increased sensitivity to different alcohols. In this work, we studied Rpn4-dependent response of different yeast strains to short-term ethanol exposure. Three S. cerevisiae strains were used: wild-type (WT), mutant strain with RPN4 gene deletion (rpn4-delta), and mutant strain with decreased proteasome activity due to PRE1 deregulation (YPL). The resistance tests demonstrated an increased sensitivity of mutant strains to ethanol compared with WT. Comparative proteomics analysis revealed significant differences in molecular responses to ethanol between different strains. GO analysis of proteins upregulated in WT showed enrichments represented by oxidative and heat responses, protein folding/unfolding and protein degradation. Enrichment of at least one of these responses was not observed in mutant strains. At the same time, trehalose synthesis and accumulation of stress granules were enhanced in the mutant strains. We suggest that these pathways could partially compensate for the failure of ubiquitin-proteasome system to cope with the ethanol stress. Also, we suggest impaired compensatory activation of autophagic degradation system in rpn4-delta strain and propose that Rpn4 can be a regulator for autophagy upon ethanol stress. These findings can be a basis for creating genetically modified yeast strains resistant to high levels of alcohol, being further used for fermentation in ethanol production.
HostingRepository	PRIDE
AnnounceDate	2020-04-22
AnnouncementXML	Submission_2020-04-21_22:04:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	julia Bubis
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-12 08:43:35	ID requested
⏵ 1	2020-04-21 22:04:16	announced

Bubis JA, Spasskaya DS, Gorshkov VA, Kjeldsen F, Kofanova AM, Lekanov DS, Gorshkov MV, Karpov VL, Tarasova IA, Karpov DS, Rpn4 and proteasome-mediated yeast resistance to ethanol includes regulation of autophagy. Appl Microbiol Biotechnol, 104(9):4027-4041(2020) [pubmed]

submitter keyword: shotgun proteomics, cell stress, S. cerevisiae, Rpn4, ethanol

Mikhail Gorshkov
contact affiliation	V.L. Talrose Institute for Energy Problems of Chemical Physics, N.N. Semenov Federal Research Center of Chemical Physics, Russian Academy of Sciences, 119334 Moscow, Russia
contact email	mike.gorshkov@gmail.com
lab head
julia Bubis
contact affiliation	V.L. Talrose Institute for Energy Problems of Chemical Physics
contact email	juliabubis@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014236
     1. Label: PRIDE project
     2. Name: Rpn4- and Proteasome- Mediated Yeast Response to Ethanol Stress: a View from a Proteome