PXD014232

PXD014232 is an original dataset announced via ProteomeXchange.

Title	Prolonged fasting/refeeding modifies proteome profile and parvalbumin expression in the fast-twitch muscle of pacu (Piaractus mesopotamicus)
Description	Skeletal muscle in fish presents a high plasticity controlled by a dynamic balance between anabolic and catabolic signaling pathways. Decreased food availability can inhibit muscle growth and trigger muscle catabolism pathways, thu promoting muscle atrophy. In contrast, anabolism may be favored during restoration of food supply, promoting the muscle growth. Considering this, we analyzed fast-twitch muscle of juvenile Piaractus mesopotamicus (pacu) submitted to a prolonged fasting (30 days) and refeeding (up to 30 days) using shotgun proteomics and gene expression analysis. The relative rate of weight and length increase, as well as the expression of mafbx and igf -1 genes, suggest that prolonged fasting caused muscle atrophy and that 30 days of refeeding led to partial compensatory growth. Shotgun proteomics analysis identified 99 proteins after fasting and 71 proteins after refeeding periods, of which 23 and 17 were differentially expressed after fasting and after 30 days of refeeding, respectively. Most of these differentially expressed proteins were related to cytoskeleton, muscle contraction and muscle metabolism. Among these, parvalbumin (PVALB), a calcium-binding protein and food allergen, was selected for further RT-qPCR analysis, which showed that pvalb mRNA was not changed after 30 days of fasting and 30 days of refeeding, but it was downregulated after 6h and 24h of refeeding. This suggests a post-transcriptional regulation of PVALB in fish muscle. In conclusion, our results suggest that muscle atrophy and partial compensatory growth caused by prolonged fasting and refeeding affected the muscle proteome and PVALB expression. Our results can contribute to the understanding of muscle anabolic and catabolic pathways in response to changes in food availability.
HostingRepository	PRIDE
AnnounceDate	2019-11-28
AnnouncementXML	Submission_2020-01-14_07:42:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Laura Gabriel Kuniyoshi
SpeciesList	scientific name: Piaractus mesopotamicus; NCBI TaxID: 42528;
ModificationList	No PTMs are included in the dataset
Instrument	Q-Tof Premier

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-12 06:18:23	ID requested
1	2019-11-28 01:44:51	announced
⏵ 2	2020-01-14 07:42:36	announced	2020-01-14: Updated publication reference for PubMed record(s): 31856193.

da Silva-Gomes RN, Gabriel Kuniyoshi ML, Oliveira da Silva Duran B, Thomazini Zanella BT, Paccielli Freire P, Gutierrez de Paula T, de Almeida Fantinatti BE, Sim, õ, es Salom, ã, o RA, Carvalho RF, Delazari Santos L, Dal-Pai-Silva M, Prolonged fasting followed by refeeding modifies proteome profile and parvalbumin expression in the fast-twitch muscle of pacu (Piaractus mesopotamicus). PLoS One, 14(12):e0225864(2019) [pubmed]

submitter keyword: muscle atrophy, fasting, refeeding, compensatory growth, parvalbumin, proteomics

Maeli Dal-Pai-Silva
contact affiliation	Department of Morphology, Institute of Biosciences of Botucatu, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
contact email	maeli.dal-pai@unesp.br
lab head
Maria Laura Gabriel Kuniyoshi
contact affiliation	IBB-UNESP
contact email	marialgk@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014232
     1. Label: PRIDE project
     2. Name: Prolonged fasting/refeeding modifies proteome profile and parvalbumin expression in the fast-twitch muscle of pacu (Piaractus mesopotamicus)