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PXD014231

PXD014231 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleVAMP7-dependent autophagic secretion allows for axonal resilience in growth restricting conditions.VAMP7-dependent autophagic secretion allows for axonal resilience in growth restricting conditions.
DescriptionVAMP7 is involved in autophagy and exocytosis mediating neurite growth, two yet unconnected cellular pathways. Here we show the occurrence of combined VAMP7/ATG9 secretory events. VAMP7 localized, together with LC3 and ATG9, in vesicles moving anterogradely along the axon towards growth cones. VAMP7 knockout disrupted the autophagy response to drugs and starvation. Release of extracellular vesicles triggered by autophagy was impaired in VAMP7-knockout cells and autophagy-deficient cells were impaired in VAMP7 exocytosis. Secretomics showed that VAMP7-knockout cells were impaired in unconventional secretion of cytoplasmic, exosomal and mitochondrial proteins. We further found that autophagy stimulated neurite growth in a VAMP7-dependent manner. Furthermore, neurons still grew long axons in nutrient-restricted conditions and when treated with autophagy-inducing drugs. A nanobody directed against VAMP7 inhibited the effect of nutrient restriction. We propose that VAMP7-dependent autophagic secretion contributes to a resilience mechanism to preserve axonal growth in restriction conditions, as part of brain sparing occurring in growth restriction.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_13:30:28.781.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChiara guerrera
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListcarbamoylated residue; (R)-5-oxo-1,4-tetrahydrothiazine-3-carboxylic acid
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-06-12 06:18:18ID requested
12024-10-07 13:30:29announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: autophagy, neuroscience, secretome
Contact List
GUERRERA Ida Chiara
contact affiliationUniversity of Paris, Proteomics platform 3P5-Necker, Université Paris Descartes-Structure Fédérative de Recherche Necker, INSERM US24/CNRS UMS3633, Paris, France.
contact emailchiara.guerrera@inserm.fr
lab head
Chiara guerrera
contact affiliationNecker proteomics, INSERM
contact emailchiara.guerrera@inserm.fr
dataset submitter
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