PXD014231

PXD014231 is an original dataset announced via ProteomeXchange.

Title	VAMP7-dependent autophagic secretion allows for axonal resilience in growth restricting conditions.VAMP7-dependent autophagic secretion allows for axonal resilience in growth restricting conditions.
Description	VAMP7 is involved in autophagy and exocytosis mediating neurite growth, two yet unconnected cellular pathways. Here we show the occurrence of combined VAMP7/ATG9 secretory events. VAMP7 localized, together with LC3 and ATG9, in vesicles moving anterogradely along the axon towards growth cones. VAMP7 knockout disrupted the autophagy response to drugs and starvation. Release of extracellular vesicles triggered by autophagy was impaired in VAMP7-knockout cells and autophagy-deficient cells were impaired in VAMP7 exocytosis. Secretomics showed that VAMP7-knockout cells were impaired in unconventional secretion of cytoplasmic, exosomal and mitochondrial proteins. We further found that autophagy stimulated neurite growth in a VAMP7-dependent manner. Furthermore, neurons still grew long axons in nutrient-restricted conditions and when treated with autophagy-inducing drugs. A nanobody directed against VAMP7 inhibited the effect of nutrient restriction. We propose that VAMP7-dependent autophagic secretion contributes to a resilience mechanism to preserve axonal growth in restriction conditions, as part of brain sparing occurring in growth restriction.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:30:28.781.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chiara guerrera
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	carbamoylated residue; (R)-5-oxo-1,4-tetrahydrothiazine-3-carboxylic acid
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-12 06:18:18	ID requested
⏵ 1	2024-10-07 13:30:29	announced

Dataset with its publication pending

submitter keyword: autophagy, neuroscience, secretome

GUERRERA Ida Chiara
contact affiliation	University of Paris, Proteomics platform 3P5-Necker, Université Paris Descartes-Structure Fédérative de Recherche Necker, INSERM US24/CNRS UMS3633, Paris, France.
contact email	chiara.guerrera@inserm.fr
lab head
Chiara guerrera
contact affiliation	Necker proteomics, INSERM
contact email	chiara.guerrera@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD014231

PRIDE project URI

• PRIDE
  1. PXD014231
     1. Label: PRIDE project
     2. Name: VAMP7-dependent autophagic secretion allows for axonal resilience in growth restricting conditions.VAMP7-dependent autophagic secretion allows for axonal resilience in growth restricting conditions.