PXD014225

PXD014225 is an original dataset announced via ProteomeXchange.

Title	Time-resolved phosphoproteomic analysis of the TCR signaling pathway in primary T cells
Description	T cell receptor (TCR) signaling is essential for the function of T cells. Here we monitor and quantify the dynamics of phosphorylation sites in primary CD4+ T cells during the first 10 minutes after antibody-based TCR stimulation. To capture the earliest molecular events induced by TCR engagement we prepared unstimulated and stimulated T cells (15, 30, 120, 300 or 600 seconds after stimulation). Cells were then lysed in urea, proteins were digested into tryptic peptides, and phosphorylated peptides were enriched using titanium (TiO2) beads. In most experiments a further enrichment step was performed using phospho-Tyrosine (pTyr) immuno-precipitation. We performed 4 independent experiments, each containing 6 time-points of stimulation. This dataset contains the raw LC-MS/MS files of the pre-enriched peptide samples (SP, 24 samples) and the matching TiO2-enriched peptides (TiO2, 24 samples), as well as the eluates from further pTyr immuno-precipitation from 3 experiments (Ptyr, replicates 1, 3, 4: 18 samples). All samples were analyzed in triplicate or quadruplicate nanoLC-MS runs.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:08:45.502.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anne Gonzalez de Peredo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-12 01:27:41	ID requested
1	2020-07-09 03:18:03	announced
⏵ 2	2024-10-22 05:08:47	announced	2024-10-22: Updated project metadata.

10.15252/msb.20209524;

Locard-Paulet M, Voisinne G, Froment C, Goncalves Menoita M, Ounoughene Y, Girard L, Gregoire C, Mori D, Martinez M, Luche H, Garin J, Malissen M, Burlet-Schiltz O, Malissen B, Gonzalez de Peredo A, Roncagalli R, LymphoAtlas: a dynamic and integrated phosphoproteomic resource of TCR signaling in primary T cells reveals ITSN2 as a regulator of effector functions. Mol Syst Biol, 16(7):e9524(2020) [pubmed]

submitter keyword: TCR, primary T cell, phosphoproteomics, signaling

Odile Schiltz
contact affiliation	Institute of Pharmacology and Structural Biology, CNRS, Toulouse
contact email	Odile.Schiltz@ipbs.fr
lab head
Anne Gonzalez de Peredo
contact affiliation	CNRS/IPBS
contact email	gonzalez@ipbs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD014225

PRIDE project URI

• PRIDE
  1. PXD014225
     1. Label: PRIDE project
     2. Name: Time-resolved phosphoproteomic analysis of the TCR signaling pathway in primary T cells