PXD014224

PXD014224 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic study on liver lipid droplets and mitochondria from mouse housed in different temperatures
Description	Laboratory mice are standardly housed around 23ºC, setting them under chronic cold stress. Metabolic changes of liver in mice housed under thermoneutral, standard and cold temperatures remain unknown. Here we isolated lipid droplets and mitochondria from their livers for comparative proteomic study to investigate the changes. According to proteomic analysis, mitochondrial TCA cycle and retinol metabolism were enhanced while oxidative phosphorylation was no obviously affected under cold condition, suggesting liver mitochondria may increase TCA cycle capacity in biosynthetic pathways as well as retinol metabolism to help liver to adapt. Based on proteomic and immunoblotting results, Perilipin 5 and major urinary proteins were increased significantly while mitochondrial pyruvate carrier was decreased dramatically under cold condition, indicating a role they may play.
HostingRepository	PRIDE
AnnounceDate	2019-07-02
AnnouncementXML	Submission_2019-07-01_23:59:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shuyan Zhang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-12 01:26:57	ID requested
⏵ 1	2019-07-01 23:59:49	announced

Liu Q, Zhou Z, Liu P, Zhang S, Comparative proteomic study of liver lipid droplets and mitochondria in mice housed at different temperatures. FEBS Lett, 593(16):2118-2138(2019) [pubmed]

submitter keyword: thermoneutral temperature, chronic cold stress, organellar proteomics, liver adaptation, lipid droplet, mitochondrion

Pingsheng Liu
contact affiliation	Institute of Biophysics, Chinese Academy of Sciences
contact email	pliu@ibp.ac.cn
lab head
Shuyan Zhang
contact affiliation	Institute of Biophysics, Chinese Academy of Sciences
contact email	syzhang@ibp.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014224
     1. Label: PRIDE project
     2. Name: Comparative proteomic study on liver lipid droplets and mitochondria from mouse housed in different temperatures