PXD014218
PXD014218 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | BioID2_BirA-MAVS_CLPB-BirA_PHB1-BirA_HEK293 |
Description | HEK293 cells that stably express MAVS, CLPB or PHB1 fused at C-terminus with the biotin ligase from A. aeolicus were incubated with 100 μM biotin for 18 h. The mitochondrial fractions were lysed in RIPA buffer, and the clarified supernatants were incubated with streptavidin agarose beads for 2 h at 4° C. Proteins on the beads were digested by adding trypsin/Lys-C mix for 16 h at 37°C and analyzed by LC-MS/MS. Label-free quantification was performed using Proteome Discoverer 2.2 software. |
HostingRepository | jPOST |
AnnounceDate | 2019-09-05 |
AnnouncementXML | Submission_2022-09-18_02:22:31.630.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hidetaka Kosako |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | L-methionine sulfoxide; alpha-amino acetylated residue; unknown modification |
Instrument | instrument |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-06-11 18:00:33 | ID requested | |
1 | 2019-09-05 02:36:39 | announced | |
2 | 2019-09-26 00:24:53 | announced | 2019-09-26: Updated PubMed. |
3 | 2021-02-04 17:32:21 | announced | 2021-02-04: Updated FTP location. |
⏵ 4 | 2022-09-18 02:22:32 | announced | 2022-09-18: Updated FTP location. |
Publication List
Yoshinaka T, Kosako H, Yoshizumi T, Furukawa R, Hirano Y, Kuge O, Tamada T, Koshiba T, Structural Basis of Mitochondrial Scaffolds by Prohibitin Complexes: Insight into a Role of the Coiled-Coil Region. iScience, 19():1065-1078(2019) [pubmed] |
Keyword List
submitter keyword: BioID |
Contact List
Hidetaka Kosako | |
---|---|
lab head | |
Hidetaka Kosako | |
contact affiliation | Tokushima University |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000618/ |