PXD014212 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Malus x domestica microshoot chloroplast proteome at different growth temperatures |
Description | Chloroplasts were purified by centrifugation in Percoll gradient from low temperature (4 C), control (22 C) and high temperature (30 C) grown Malus x domestica microshoots. The proteins were digested using filter aided sample processing (FASP) and analyzed using nano-LC MS/MS. Constant scan rate of 2 Hz and range from 100 to 2,200 m/z was used for the MS spectra acquisition. Data-dependent auto-MS/MS method (Compass 1.5 acquisition and processing software, Bruker Daltonics) was used for selecting the 10 most abundant precursor ions for fragmentation. The MS/MS summation time was adjusted based on precursor intensity within the range 1-10 Hz. Active exclusion of already analyzed precursors was used with a release time of 30 s. Precursor ion isolation window and the CID collision energy were adjusted between 3-9 Th and 34-55 eV as a function of the m/z value, respectively. The data files (9 low temperature samples, 12 control samples and 9 high temperature sampes) are described in the "metadata.txt" file. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:52:07.752.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Perttu Haimi |
SpeciesList | scientific name: Malus domestica; NCBI TaxID: 3750; |
ModificationList | acetylated residue |
Instrument | maXis |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-06-11 05:12:36 | ID requested | |
1 | 2019-10-03 04:20:11 | announced | |
⏵ 2 | 2024-10-22 04:52:13 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1002/pmic.201800142; |
Mork, ū, nait, ė, -Haimi Š, Vinskiene J, Stanien, ė G, Haimi P, Differential Chloroplast Proteomics of Temperature Adaptation in Apple (Malus x domestica Borkh.) Microshoots. Proteomics, 19(19):e1800142(2019) [pubmed] |
Keyword List
submitter keyword: temperature,Malus x domestica, chloroplast |
Contact List
Perttu Haimi |
contact affiliation | Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry |
contact email | perttu.haimi@gmail.com |
lab head | |
Perttu Haimi |
contact affiliation | Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry |
contact email | perttu.haimi@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014212
- Label: PRIDE project
- Name: Malus x domestica microshoot chloroplast proteome at different growth temperatures