PXD014212

PXD014212 is an original dataset announced via ProteomeXchange.

Title	Malus x domestica microshoot chloroplast proteome at different growth temperatures
Description	Chloroplasts were purified by centrifugation in Percoll gradient from low temperature (4 C), control (22 C) and high temperature (30 C) grown Malus x domestica microshoots. The proteins were digested using filter aided sample processing (FASP) and analyzed using nano-LC MS/MS. Constant scan rate of 2 Hz and range from 100 to 2,200 m/z was used for the MS spectra acquisition. Data-dependent auto-MS/MS method (Compass 1.5 acquisition and processing software, Bruker Daltonics) was used for selecting the 10 most abundant precursor ions for fragmentation. The MS/MS summation time was adjusted based on precursor intensity within the range 1-10 Hz. Active exclusion of already analyzed precursors was used with a release time of 30 s. Precursor ion isolation window and the CID collision energy were adjusted between 3-9 Th and 34-55 eV as a function of the m/z value, respectively. The data files (9 low temperature samples, 12 control samples and 9 high temperature sampes) are described in the "metadata.txt" file.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:52:07.752.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Perttu Haimi
SpeciesList	scientific name: Malus domestica; NCBI TaxID: 3750;
ModificationList	acetylated residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-11 05:12:36	ID requested
1	2019-10-03 04:20:11	announced
⏵ 2	2024-10-22 04:52:13	announced	2024-10-22: Updated project metadata.

10.1002/pmic.201800142;

Mork, ū, nait, ė, -Haimi Š, Vinskiene J, Stanien, ė G, Haimi P, Differential Chloroplast Proteomics of Temperature Adaptation in Apple (Malus x domestica Borkh.) Microshoots. Proteomics, 19(19):e1800142(2019) [pubmed]

submitter keyword: temperature,Malus x domestica, chloroplast

Perttu Haimi
contact affiliation	Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry
contact email	perttu.haimi@gmail.com
lab head
Perttu Haimi
contact affiliation	Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry
contact email	perttu.haimi@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD014212

PRIDE project URI

• PRIDE
  1. PXD014212
     1. Label: PRIDE project
     2. Name: Malus x domestica microshoot chloroplast proteome at different growth temperatures