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PXD014212

PXD014212 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMalus x domestica microshoot chloroplast proteome at different growth temperatures
DescriptionChloroplasts were purified by centrifugation in Percoll gradient from low temperature (4 C), control (22 C) and high temperature (30 C) grown Malus x domestica microshoots. The proteins were digested using filter aided sample processing (FASP) and analyzed using nano-LC MS/MS. Constant scan rate of 2 Hz and range from 100 to 2,200 m/z was used for the MS spectra acquisition. Data-dependent auto-MS/MS method (Compass 1.5 acquisition and processing software, Bruker Daltonics) was used for selecting the 10 most abundant precursor ions for fragmentation. The MS/MS summation time was adjusted based on precursor intensity within the range 1-10 Hz. Active exclusion of already analyzed precursors was used with a release time of 30 s. Precursor ion isolation window and the CID collision energy were adjusted between 3-9 Th and 34-55 eV as a function of the m/z value, respectively. The data files (9 low temperature samples, 12 control samples and 9 high temperature sampes) are described in the "metadata.txt" file.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:52:07.752.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPerttu Haimi
SpeciesList scientific name: Malus domestica; NCBI TaxID: 3750;
ModificationListacetylated residue
InstrumentmaXis
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-06-11 05:12:36ID requested
12019-10-03 04:20:11announced
22024-10-22 04:52:13announced2024-10-22: Updated project metadata.
Publication List
10.1002/pmic.201800142;
Mork, ū, nait, ė, -Haimi Š, Vinskiene J, Stanien, ė G, Haimi P, Differential Chloroplast Proteomics of Temperature Adaptation in Apple (Malus x domestica Borkh.) Microshoots. Proteomics, 19(19):e1800142(2019) [pubmed]
Keyword List
submitter keyword: temperature,Malus x domestica, chloroplast
Contact List
Perttu Haimi
contact affiliationInstitute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry
contact emailperttu.haimi@gmail.com
lab head
Perttu Haimi
contact affiliationInstitute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry
contact emailperttu.haimi@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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