PXD014208

PXD014208 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic analysis of lettuce (Lactuca sativa L.) reveals starch and sucrose metabolism functions during bolting induced by high temperature
Description	Temperature plays an important role in the growth and development of lettuce (Lactuca sativa L.). A high temperature can induce bolting, which decreases both the quality and production of lettuce. However, knowledge of underlying lettuce bolting is still lacking, especially in the molecular basis that high temperature promotes the premature bolting of lettuce at the phosphorylated level. In this study, we analyzed the lettuce stems during the bolting period induced by a high temperature (33oC/25oC, day/ night) and a controlled temperature (20oC/13oC, day/night) using iTRAQ-based phosphoproteome and 987 phosphoproteins were significantly changed after high-temperature treatment and a total of 3814 phosphorylation sites located in 1766 phosphopeptides were identified. Also, we found 216 phosphoproteins significantly regulated, of which 116 up-regulated and 101 down-regulated, and the abundance changed peptides after high-temperature treatment was significantly involved in the biological process (BP) of water homeostasis. Most of these phosphoproteins were associated with metabolic process, correspondingly the main molecular functions are catalytic activity and transporter activity. The top 10 signaling pathways included spliceosome, starch and sucrose metabolism, RNA transport, protein processing in endoplasmic reticulum, phosphatidylinositol signaling system, phospholipase D signaling pathway, apoptosis, protein export, tight junction, and glycerolipid metabolism. For further, we found the high-temperature influence phosphoprotein activity associated with metabolism, especially, starch and sucrose metabolism, glycerolipid metabolism, methane metabolism. The kinases, including proline-directed, acidic, basic, others and tyrosine, were predicted to be involved in signal transduction and metabolism regulation. The proline-directed motif such as ..SP..[s]P, [s]P, [t]P was the dominant class, proline-directed motifs associated with MAK kinase, and several acidic motifs are casein kinase II motif are necessary for lettuce blotting. Our study showed phosphoproteome profiling in response to high temperature and provides a better understanding of the molecular mechanisms regulated in lettuce blotting
HostingRepository	iProX
AnnounceDate	2019-06-10
AnnouncementXML	Submission_2023-03-01_19:42:36.064.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lifeng Meng
SpeciesList	scientific name: Lactuca sativa var. capitata; NCBI TaxID: 1049369;
ModificationList	phosphorylated residue
Instrument	Thermo Fisher Scientific instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-10 19:37:39	ID requested
1	2019-06-10 19:41:16	announced
⏵ 2	2023-03-01 19:42:37	announced	2023-03-02: Update publication information.

Qin X, Li P, Lu S, Sun Y, Meng L, Hao J, Fan S, Phosphoproteomic analysis of lettuce (Lactuca sativa L.) reveals starch and sucrose metabolism functions during bolting induced by high temperature. PLoS One, 15(12):e0244198(2020) [pubmed]

submitter keyword: lettuce, bolting, iTRAQ, phosphoprotein, kinases

Jianke Li
contact affiliation	Insititute of Apiculture Reseach
contact email	apislijk@126.com
lab head
Lifeng Meng
contact affiliation	Insititute of Apiculture Reseach, Chinese Academy of Agricultural Science
contact email	menglifeng@caas.cn
dataset submitter

iProX dataset URI