PXD014205

PXD014205 is an original dataset announced via ProteomeXchange.

Title	Evolution Anaerobic peroxisome in Mastigamoeba balamuthi
Description	Adaptation of eukaryotic cells to anaerobic condition is reflected by deep changes in mitochondrial metabolism and their functional reduction. The most modified types of mitochondria are hydrogenosomes that generate molecular hydrogen with concomitant ATP synthesis and mitosome that completely lost energy metabolism. The reduction of mitochondria is associated with loss of peroxisomes that evolved from ER to compartmentalize pathways generating reactive oxygen species (ROS) and thus prevent cellular oxidative damage. Biogenesis and function of peroxisomes is tightly coupled with mitochondria. They share the fission machinery, pathways of oxidative metabolism, ROS scavenging, and metabolic products. The loss of peroxisomes in anaerobic eukaryotes with reduced mitochondria is thus not unexpected. Surprisingly, we identified peroxisomes in anaerobic, hydrogenosome bearing protist Mastigamoeba balamuthi. Initially, we identified conserved set of peroxisomal proteins peroxins that are required for protein import, peroxisomal growth and division. Key membrane associated peroxins (MbPex3, MbPex11, and MbPex14) were visualized in numerous vesicles that were distinct from hydrogenosomes, ER and Golgi body. Proteomic analysis of cellular fractions and prediction of peroxisomal targeting signals (PTS1/PTS2) allows identification of 51 putative peroxisomal matrix proteins. Expression of selected proteins in Saccharomyces cerevisiae revealed that they are specifically targeted to yeast peroxisomes. Matrix protein includes components of acyl CoA and carbohydrate metabolism, pyrimidine and CoA biosynthesis, whereas neither components of β-oxidation nor catalase were present. In conclusion, we identified new subclass of peroxisomes named “anaerobic” peroxisome that shifts the current paradigm and rises attention to reductive evolution of peroxisomes in anaerobic organisms.
HostingRepository	PRIDE
AnnounceDate	2020-03-04
AnnouncementXML	Submission_2020-03-04_04:01:35.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014205
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Karel Harant
SpeciesList	scientific name: Mastigamoeba balamuthi ATTC 30984; NCBI TaxID: 1313166;
ModificationList	Methylthio; TMT6plex; Oxidation
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-10 08:26:51	ID requested
⏵ 1	2020-03-04 04:01:37	announced

Le T, Ž, á, rsk, ý V, N, ý, vltov, á E, Rada P, Harant K, Vancov, á M, Verner Z, Hrd, ý I, Tachezy J, . Proc Natl Acad Sci U S A, 117(4):2065-2075(2020) [pubmed]

submitter keyword: Peroxisome, Mastigamoeba balamuthi, anaerobiosis

Jan Tachezy
contact affiliation	Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Průmyslová 595, 25242 Vestec, Czech Republic
contact email	jan.tachezy@natur.cuni.cz
lab head
Karel Harant
contact affiliation	Charles University
contact email	harant@natur.cuni.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014205
     1. Label: PRIDE project
     2. Name: Evolution Anaerobic peroxisome in Mastigamoeba balamuthi