PXD014202

PXD014202 is an original dataset announced via ProteomeXchange.

Title	Identification of desuccinylase ScCobB2 and its desuccinylation substrates in Streptomyces coelicolor
Description	As a recently discovered protein posttranslational modification in eukaryotes, lysine succinylation has attracted increasing interest due to its ability to regulate several critical cellular processes including catabolism, β-oxidation, and ketogenesis. Nevertheless, understanding of the regulation mechanism is still at an early stage due to the lack of identified specific desuccinylases in most microorganisms.Here, in the model soil bacteria Streptomyces coelicolor, we biochemically characterized a sirtuin-like protein ScCobB2 as a divergent desuccinylase. Based on it, we identified a total of 673 unique succinylated sites, of which 470 sites in 317 proteins were quantified by combining tandem mass tag labeling, antibody affinity enrichment, and LC−MS/MS analysis. Furtheranalyses of the quantitative succinylome revealed that at least 114 proteins representing two major pathways, carbon metabolism and protein biosynthesis, are obviously hypersuccinylated in ∆ScCobB2 cells. We experimentally examined the regulatory roles of ScCobB2 on 13 hypersuccinylated proteins, including glyceraldehyde-3-phosphate dehydrogenase, aconitate hydratase, and several ribosomal proteins, the results of which suggested a high confidence in our quantitative data. This work provided the first discovery of a specificdesuccinylase in bacteria, and demonstrated it has pivotal regulatory role in multiple biological processes for S. coelicolor, laid the foundation for future research of succinylation regulation in other microorganisms.
HostingRepository	PRIDE
AnnounceDate	2019-06-11
AnnouncementXML	Submission_2019-07-30_07:04:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Zhao
SpeciesList	scientific name: Streptomyces coelicolor; NCBI TaxID: 1902;
ModificationList	N6-succinyl-L-lysine
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-10 06:39:59	ID requested
1	2019-06-10 23:58:55	announced
⏵ 2	2019-07-30 07:04:36	announced	Updated publication reference for PubMed record(s): 31337674.

Zhang H, Li P, Ren S, Cheng Z, Zhao G, Zhao W, . Mol Cell Proteomics, 18(10):2003-2017(2019) [pubmed]

submitter keyword: post-translational modification

desuccinylase

ScCobB2

lysine succinylation

succinylome

Wei Zhao
contact affiliation	Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
contact email	weizhao008@gmail.com
lab head
Wei Zhao
contact affiliation	Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
contact email	181zhao@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014202
     1. Label: PRIDE project
     2. Name: Identification of desuccinylase ScCobB2 and its desuccinylation substrates in Streptomyces coelicolor