PXD014200 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Exploring the HeLa Dark Mitochondrial proteome |
| Description | In the framework of the Human Proteome Project initiative, we aim to improve mapping and characterization of mitochondrial proteome. In this work we implemented an experimental workflow, combining classical biochemical enrichments and mass spectrometry, to pursue a much deeper definition of mitochondrial proteome and possibly mine mitochondrial uncharacterized
dark proteins. We fractionated in two compartments mitochondria enriched from HeLa cells in order to annotate 4230 proteins in both fraction by means of a multiple-enzyme digestion (trypsin, chymotrypsin and Glu-C) followed by mass spectrometry analysis using a combination of Data Dependent Acquisition and Data Independent Acquisition. We detected 22 mitochondrial dark proteins not annotated for their function and we provide their relative abundance inside the mitochondrial organelle. Considering this work as a pilot study we expect that the same approach, in different biological system, could represent an advancement in the characterization of the human mitochondrial proteome providing uncharted ground to explore the mitonuclear phenotypic
relationships.
All spectra have been deposited to ProteomeXchange with PXD014201 and PXD014200 identifier.
|
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_04:55:51.715.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Federica Marini |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Synapt MS |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2019-06-10 05:11:23 | ID requested | |
| 1 | 2020-02-27 08:20:46 | announced | |
| 2 | 2020-02-27 22:37:33 | announced | 2020-02-28: Updated publication reference for DOI(s): 10.3389/fcell.2020.00137. |
| 3 | 2023-11-14 08:50:37 | announced | 2023-11-14: Updated project metadata. |
| ⏵ 4 | 2024-10-22 04:55:52 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
| ProteomeXchange project tag: Human Proteome Project, Mitochondria (HPP) |
| submitter keyword: personalised medicine, subproteome, mass spectrometry, mitochondria, drug targets,dark proteome |
Contact List
| Luisa Pieroni |
|---|
| contact affiliation | Proteomics and Metabonomics Unit Fondazione Santa Lucia-IRCCS Via del Fosso di Fiorano 64, 00145 Rome, Italy |
| contact email | l.pieroni@hsantalucia.it |
| lab head | |
| Federica Marini |
|---|
| contact affiliation | Catholic University of Sacred Heart (Rome) |
| contact email | federica.marini@unicatt.it |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/02/PXD014200 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014200
- Label: PRIDE project
- Name: Exploring the HeLa Dark Mitochondrial proteome
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