PXD014191

PXD014191 is an original dataset announced via ProteomeXchange.

Title	Proteome profiling of the exhaled breath condensate after long-term spaceflights
Description	Abstract: The aim of the study was to analyze exhaled breath condensate (EBC) proteome changes due to the effects of spaceflight factors. All the protocols were approved at the Institute of Biomedical Problems – Russian Federation State Scientific Research Center in the frame of «Protocon» experiment. EBC samples were collected by commercial devices RTube one month before flight (background) at the Yu. A. Gagarin Research and Test Cosmonaut Training Center, immediately after landing of the landing modules in the field (R0), and on the seventh day after landing as a part of medical examination (R+7). Semi-quantitative label-free proteomic analysis of 13 EBC samples collected from 5 Russian cosmonauts before and after long-term (169–199 days) spaceflights were performed and resulted in 164 different proteins. The highest number of proteins was detected in EBC after landing (R0). Pathways enrichment analysis via GO database large group of proteins that take part in keratinization processes (CASP14, DSG1, DSP, JUP, and etc). Nine proteins were KRT2, KRT9, KRT1, KRT10, KRT14, DCD, KRT6C, KRT6A, KRT5 were detected in all groups (background, R0, R+1). A two-sample Welch’s t-test identified the significant changing of KRT2 and KRT9 levels after landing. Enrichment analysis via KEGG database revealed significant participation (2,07E-06) of detected proteins in pathogenic E. coli infection (ACTG1, TUBA1C, TUBA4A, TUBB, TUBB8, YWHAZ). Presumably, presents of this proteins can associated with changing of cosmonauts’ microbial composition. Thus EBC can be used for noninvasive monitoring of health status and respiratory tract pathologies during the spaceflight. The obtained data are important for the development of medicine in extreme situations.
HostingRepository	PRIDE
AnnounceDate	2019-09-27
AnnouncementXML	Submission_2019-09-27_15:09:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexander Brzhozovskiy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-10 01:24:59	ID requested
⏵ 1	2019-09-27 15:09:34	announced

Kononikhin AS, Brzhozovskiy AG, Ryabokon AM, Fedorchenko K, Zakharova NV, Spasskii AI, Popov IA, Ilyin VK, Solovyova ZO, Pastushkova LK, Polyakov AV, Varfolomeev SD, Larina IM, Nikolaev EN, Proteome Profiling of the Exhaled Breath Condensate after Long-Term Spaceflights. Int J Mol Sci, 20(18):(2019) [pubmed]

submitter keyword: EBC, exhaled breath condensate

Irina Larina
contact affiliation	IBP RAS
contact email	irina.larina@gmail.com
lab head
Alexander Brzhozovskiy
contact affiliation	V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia
contact email	agb.imbp@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD014191

PRIDE project URI

• PRIDE
  1. PXD014191
     1. Label: PRIDE project
     2. Name: Proteome profiling of the exhaled breath condensate after long-term spaceflights