PXD014189

PXD014189 is an original dataset announced via ProteomeXchange.

Title	The heat shock response in yeast is modular and maintains protein homeostasis via distinct mechanisms
Description	We globally analyzed the heat stress response (HSR) in yeast on the transcriptomic, tranlatomic and the proteomic level. The cells were stressed at 37 ??????C, 42 ??????C and 46 ??????C and the fractionated proteomes measured on a Fusion and Q-Exactive Plus (both Thermo) mass spectrometer, respectively. With this approach we were able to reveal a modular character of the HSR dependent on the severity of the stress. We showed that at temperatures up to 42 ??????C besides upregulation of stress protective factors refilling the pool of degraded and aggregating proteins is an important branch of the HSR. Whereas, at sublethal temperatures (46 ??????C) lots of aggregation and phase transition processes were observed.
HostingRepository	PRIDE
AnnounceDate	2020-01-02
AnnouncementXML	Submission_2020-01-02_08:29:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Moritz M??????hlhofer
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-10 01:24:42	ID requested
1	2020-01-02 08:25:31	announced
⏵ 2	2020-01-02 08:29:03	announced	2020-01-02: Updated publication reference for PubMed record(s): 31875563.

M, ü, hlhofer M, Berchtold E, Stratil CG, Csaba G, Kunold E, Bach NC, Sieber SA, Haslbeck M, Zimmer R, Buchner J, The Heat Shock Response in Yeast Maintains Protein Homeostasis by Chaperoning and Replenishing Proteins. Cell Rep, 29(13):4593-4607.e8(2019) [pubmed]

submitter keyword: heat shock response, phase transition, chaperones, proteome, LFQ, SILAC

Johannes Buchner
contact affiliation	Technische Universit??????t M??????nchen, Dept. Chemistry, Chair of Biotechnology
contact email	johannes.buchner@tum.de
lab head
Moritz M??????hlhofer
contact affiliation	Dept. Chemie Lichtenbergstra??????e 4 85748 Garching
contact email	moritz.muehlhofer@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014189
     1. Label: PRIDE project
     2. Name: The heat shock response in yeast is modular and maintains protein homeostasis via distinct mechanisms