PXD014187

PXD014187 is an original dataset announced via ProteomeXchange.

Title	Effect of Nop53 depletion on the exosome interactome
Description	In order to evaluate how Nop53 modulates the interaction of Rrp6 and Rrp43 with other factors, a coimmunoprecipitation assay was performed both in the presence (-doxicycline) and upon depletion (+ doxicycline) of Nop53 using the condional strain Δnop53/tetOff::GFP-NOP53 carrying the Rrp6-TAP or Rrp43-TAP fusion. As a negative control, the same strain carrying only the TAP tag was employed. For each condition, the elution of biological triplicates was subjected to label free quantitative analysis.
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_11:15:11.783.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Felipe Bagatelli
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-10 01:24:10	ID requested
⏵ 1	2021-09-08 11:15:12	announced

Cepeda LPP, Bagatelli FFM, Santos RM, Santos MDM, Nogueira FCS, Oliveira CC, The ribosome assembly factor Nop53 controls association of the RNA exosome with pre-60S particles in yeast. J Biol Chem, 294(50):19365-19380(2019) [pubmed]

submitter keyword: Nop53, Ribosome biogenesis, RNA exosome, rRNA processing

Carla C. Oliveira
contact affiliation	Department of Biochemistry, Institute of Chemistry, University of São Paulo,São Paulo, SP, Brazil
contact email	ccoliv@iq.usp.br
lab head
Felipe Bagatelli
contact affiliation	Univestity of Sao Paulo
contact email	felipe.bagatelli@usp.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014187
     1. Label: PRIDE project
     2. Name: Effect of Nop53 depletion on the exosome interactome