PXD014174

PXD014174 is an original dataset announced via ProteomeXchange.

Title	Beta-glucan is a major growth substrate for human gut bacteria related to Coprococcus eutactus
Description	A clone encoding carboxymethylcellulase activity was isolated during functional screening of a human gut metagenomic library using Lactococcus lactis MG1363 as heterologous host. The insert sequence revealed a glycoside hydrolase family 9 (GH9) catalytic domain with sequence similarity to a gene from Coprococcus sp. ART55/1, which is closely related to Coprocococcus eutactus. Surveys of available genomes indicated a limited distribution of GH9 domains among dominant human colonic anaerobes. Genomes of two Coprococcus-related strains showed the presence of two GH9-encoding and four GH5-encoding genes, however, the strains did not appear to be able to degrade cellulose. Instead, they grew well on beta-glucans and one of the strains also showed growth on galactomannan, galactan and glucomannan. Gene expression and proteomic analysis of Coprococcus sp. ART55/1 grown on cellobiose, beta-glucan and lichenan led to a similar change in expression in comparison to glucose. On beta-glucan and lichenan only, one of the four GH5 genes was strongly upregulated. Growth on glucomannan lead to a transcriptional response of many genes, in particular a strong upregulation of glycoside hydrolases involved in mannan degradation. Thus, beta-glucans are a major growth substrate for species related to Coprococcus eutactus, with glucomannan and galactans alternative substrates for some strains.
HostingRepository	PRIDE
AnnounceDate	2020-03-10
AnnouncementXML	Submission_2020-03-10_04:14:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sophie Shaw
SpeciesList	scientific name: Coprococcus sp. ART55/1; NCBI TaxID: 751585;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-07 03:52:59	ID requested
⏵ 1	2020-03-10 04:14:45	announced

Alessi AM, Gray V, Farquharson FM, Flores-L, ó, pez A, Shaw S, Stead D, Wegmann U, Shearman C, Gasson M, Collie-Duguid ESR, Flint HJ, Louis P, -Glucan is a major growth substrate for human gut bacteria related to Coprococcus eutactus. Environ Microbiol, 22(6):2150-2164(2020) [pubmed]

submitter keyword: Coprococcus eutactus

beta-glucan;

Petra Louis
contact affiliation	Rowett Institute Gut Health Theme University of Aberdeen Foresterhill Aberdeen AB25 2ZD
contact email	p.louis@abdn.ac.uk
lab head
Sophie Shaw
contact affiliation	University of Aberdeen
contact email	s.shaw@abdn.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD014174

PRIDE project URI

• PRIDE
  1. PXD014174
     1. Label: PRIDE project
     2. Name: Beta-glucan is a major growth substrate for human gut bacteria related to Coprococcus eutactus