PXD014171

PXD014171 is an original dataset announced via ProteomeXchange.

Title	A window into disease: Protein signatures of remodeled airways in transplanted lungs with Bronchiolitis Obliterans Syndrome
Description	Background: Bronchiolitis obliterans syndrome (BOS), the most common form of chronic lung allograft dysfunction (CLAD), is the major limitation to long-term survival after lung transplantation. The patho-anatomical correlate is progressive, fibrotic occlusion of the small airways, so-called obliterative bronchiolitis (OB) –lesions, ultimately leading to organ failure. The molecular composition of these lesions is unknown. Methods: By combining laser-capture microdissection (LCM) and optimized sample preparation protocols for mass spectrometry (MS) we analyzed end-stage OB-lesions identified in explanted lungs from four end-stage BOS patients. Immunohistochemistry and immunofluorescence were used to follow selected proteins of interest on the tissue level. Results: We established protein signatures for in total 15 OB-lesions. A set of 12 proteins identified in all lesions included both distinct structural proteins (collagen type IV and VI) and cellular components (actins, vimentin, tryptase). Each respective lesion exhibited a unique composition of proteins (on average n=66), thereby mirroring the histomorphological variation of the lesions. Conclusions: Even though being distinct and focal pathologic events, OB-lesions showed considerable variations in their protein content, most likely reflecting different disease pathways. Combining spatial information and advanced protein identification, this study provides molecular and morphological insights essential for a better understanding of the development of chronic rejection after lung transplantation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:22:08.671.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emma Åhrman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-07 03:14:33	ID requested
1	2021-05-19 07:25:57	announced
⏵ 2	2024-10-22 05:22:09	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: chronic rejection, laser-capture microdissection, mass spectrometry, molecular profiling, lung transplantation, obliterative bronchiolitis,Bronchiolitis Obliterans Syndrome

Johan Malmstrom
contact affiliation	Professor Johan Malmstrom Infection Medicine Proteomics BMC D13 Lund University Lund Sweden
contact email	johan.malmstrom@med.lu.se
lab head
Emma Åhrman
contact affiliation	Lund University
contact email	emma.ahrman@med.lu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014171
     1. Label: PRIDE project
     2. Name: A window into disease: Protein signatures of remodeled airways in transplanted lungs with Bronchiolitis Obliterans Syndrome