PXD014153

PXD014153 is an original dataset announced via ProteomeXchange.

Title	The mechanism of infochemicals induced colony formation and flocculation in the microalga Scenedesmus subspicatus
Description	Daphnia infochemicals can induce defensive responses in microalgae Scenedesmus spp., including colony formation and flocculation. We investigated the phenomenon using a combination of microalgal biology, physiological ecology, and quantitative isobaric tagging (iTRAQ) proteomics. We have discovered that flocculation as a defense response occurs at the early ‘alarm’ phase and requires increased energy resources, with an important role proposed in cysteine synthesis. It appears to be initially stimulated by the production of an extracellular matrix (mainly composed ofby polysaccharides and fatty acids), and later sustained at the ‘acclimation’ stage through Mitogen-activated protein kinase (MAPK) signalling cascades. Defensive strategies leading to colony formation required investment into fatty acids metabolism, to ensure the accurate separation of membranes during cell division. Furthermore, higher energy demands were required at the alarm phase which subsequently decreased at the acclimation stage, thus suggesting a trade-off between colony formation and support of floc form.
HostingRepository	PRIDE
AnnounceDate	2020-05-06
AnnouncementXML	Submission_2020-06-01_14:48:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sebastiana Roccuzzo
SpeciesList	scientific name: Desmodesmus subspicatus; NCBI TaxID: 104105;
ModificationList	methylthiolated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-06 02:28:54	ID requested
1	2020-05-06 01:29:30	announced
⏵ 2	2020-06-01 14:48:56	announced	2020-06-01: Updated publication reference for PubMed record(s): 32457714.

Roccuzzo S, Couto N, Karunakaran E, Kapoore RV, Butler TO, Mukherjee J, Hansson EM, Beckerman AP, Pandhal J, Unraveled by Quantitative Proteomics. Front Microbiol, 11():792(2020) [pubmed]

submitter keyword: Daphnia infochemicals, Scenedesmus, induced defences, predation, flocculation, colony formation iTRAQ proteomics

Jagroop Pandhal
contact affiliation	Department of Chemical and Biological Engineering University of Sheffield Mappin Street, Sheffield S1 3JD United Kingdom
contact email	j.pandhal@sheffield.ac.uk
lab head
Sebastiana Roccuzzo
contact affiliation	Faculty of Environment, J. E. Purkyne University in Usti-nad-Labem
contact email	drnellaroccuzzo@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014153
     1. Label: PRIDE project
     2. Name: The mechanism of infochemicals induced colony formation and flocculation in the microalga Scenedesmus subspicatus