PXD014142

PXD014142 is an original dataset announced via ProteomeXchange.

Title	EOMES interacts with RUNX3 and BRG1 to promote innate memory cell formation through epigenetic reprogramming
Description	Memory CD8+ T cells have the ability to provide lifelong immunity against pathogens. Although memory features generally arise after challenge with a foreign antigen, naïve CD8 single positive (SP) thymocytes may acquire phenotypic and functional characteristics of memory cells in response to cytokines such as interleukin-4. This process is associated with the induction of the T-box transcription factor Eomesodermin (EOMES). However, the underlying molecular mechanisms remain ill-defined. Using epigenomic profiling, we show that these innate memory CD8SP cells acquire only a portion of the active enhancer repertoire of conventional memory cells. This reprograming is secondary to EOMES recruitment, mostly to RUNX3-bound enhancers. Furthermore, EOMES is found within chromatin-associated complexes containing BRG1 and promotes the recruitment of this chromatin remodelling factor. Also, the in vivo acquisition of EOMES-dependent program is BRG1-dependent. In conclusion, our results support a strong epigenetic basis for the EOMES-driven establishment of CD8+ T cell innate memory program.
HostingRepository	PRIDE
AnnounceDate	2019-06-06
AnnouncementXML	Submission_2019-08-01_04:36:22.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014142
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Abdulkader AZOUZ
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Ammonia-loss; Deamidated; Dehydrated; Oxidation; Dioxidation; Acetyl; Carbamidomethyl
Instrument	Thermo Scientific instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-05 09:00:24	ID requested
1	2019-06-06 00:26:24	announced
⏵ 2	2019-08-01 04:36:23	announced	Updated publication reference for PubMed record(s): 31341159.

Istaces N, Splittgerber M, Lima Silva V, Nguyen M, Thomas S, Le A, Achouri Y, Calonne E, Defrance M, Fuks F, Goriely S, Azouz A, EOMES interacts with RUNX3 and BRG1 to promote innate memory cell formation through epigenetic reprogramming. Nat Commun, 10(1):3306(2019) [pubmed]

submitter keyword: Eomes Brg1 Runx3

Stanislas GORIELY
contact affiliation	Université Libre de Bruxelles Institute for Medical Immunology
contact email	stgoriel@ulb.ac.be
lab head
Abdulkader AZOUZ
contact affiliation	Université Libre de Bruxelles
contact email	aazouz@ulb.ac.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014142
     1. Label: PRIDE project
     2. Name: EOMES interacts with RUNX3 and BRG1 to promote innate memory cell formation through epigenetic reprogramming