PXD014136

PXD014136 is an original dataset announced via ProteomeXchange.

Title	Changes to SUMO2 conjugation during Influenza virus infection of cultured cells
Description	Dynamic SUMO modifications of diverse cellular protein groups are critical to orchestrate resolution of stresses such as genome damage, hypoxia or proteotoxicity. Defense against pathogen insult (usually reliant upon host recognition of ‘non-self’ nucleic acids), is also modulated by SUMO, but the underlying mechanisms are incompletely understood. Here, we used quantitative SILAC-based proteomics to survey pan-viral host SUMOylation responses, creating a resource of almost 600 common and unique SUMO remodeling events that are mounted during influenza A and B virus infections, as well as during viral immune stimulation. By integrating knock-out/reconstitution models and transcriptomics, we provide evidence that influenza virus triggered loss of SUMO-modified TRIM28 leads to derepression of endogenous retroviral elements, unmasking this cellular pool of ‘self’ double-stranded (ds) RNA. Consequently, loss of SUMO-modified TRIM28 potentiates canonical cytosolic dsRNA-activated interferon-mediated defenses. Our data suggest that a key nuclear mechanism that prevents expression of endogenous retroviruses has been functionally co-opted via a stress-induced SUMO switch to augment antiviral immunity.
HostingRepository	PRIDE
AnnounceDate	2019-08-12
AnnouncementXML	Submission_2019-08-12_07:53:49.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mike Tatham
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	N-acetylated residue; oxidized residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-05 01:56:06	ID requested
⏵ 1	2019-08-12 07:53:50	announced

Schmidt N, Domingues P, Golebiowski F, Patzina C, Tatham MH, Hay RT, Hale BG, An influenza virus-triggered SUMO switch orchestrates co-opted endogenous retroviruses to stimulate host antiviral immunity. Proc Natl Acad Sci U S A, 116(35):17399-17408(2019) [pubmed]

submitter keyword: Influenza, SUMO, NS1

Ronald Thomas Hay
contact affiliation	Professor of Molecular Biology Centre for Gene Regulation and Expression School of Life Sciences, University of Dundee, Dundee
contact email	r.t.hay@dundee.ac.uk
lab head
Mike Tatham
contact affiliation	University of Dundee
contact email	m.tatham@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014136
     1. Label: PRIDE project
     2. Name: Changes to SUMO2 conjugation during Influenza virus infection of cultured cells