PXD014133

PXD014133 is an original dataset announced via ProteomeXchange.

Title	Global lysine crotonylation and 2-hydroxyisobutyrylation in phenotypically different Toxoplasma gondii parasites
Description	Toxoplasma gondii is a unicellular protozoan parasite of the phylum Apicomplexa. The parasite repeatedly goes through a cycle of invasion, division and induction of host cell rupture, which is an obligatory process for proliferation inside warm-blooded animals. It is known that the biology of the parasite is controlled by a variety of mechanisms ranging from genomic to epigenetic to transcriptional regulation. In this study, we investigated the global protein posttranslational lysine crotonylation and 2-hydroxyisobutyrylation of two T. gondii strains, RH and ME49, which represent distinct phenotypes for proliferation and pathogenicity in the host. Proteins with differential expression and modification patterns associated with parasite phenotypes were identified. Many proteins in T. gondii were crotonylated and 2-hydroxyisobutyrylated, and they were localized in diverse subcellular compartments involved in a wide variety of cellular functions such as motility, host invasion, metabolism and epigenetic gene regulation. These findings suggest that lysine crotonylation and 2-hydroxyisobutyrylation are ubiquitous throughout the T. gondii proteome, regulating critical functions of the modified proteins. These data provide a basis for identifying important proteins associated with parasite development and pathogenicity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:55:24.741.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qijun Chen
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: 5811;
ModificationList	N6-crotonyl-L-lysine; acetylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-05 01:55:15	ID requested
1	2019-09-09 13:15:18	announced
⏵ 2	2024-10-22 04:55:32	announced	2024-10-22: Updated project metadata.

Yin D, Jiang N, Zhang Y, Wang D, Sang X, Feng Y, Chen R, Wang X, Yang N, Chen Q, Parasites. Mol Cell Proteomics, 18(11):2207-2224(2019) [pubmed]

10.1074/mcp.ra119.001611;

submitter keyword: metabolism, protein posttranslational modification,Toxoplasma gondii, function, crotonylation, 2-hydroxyisobutyrylation

Qijun Chen
contact affiliation	Shenyang Agricultural University, 120 Dongling Road, Shenyang 110166, China
contact email	qijunchen759@syau.edu.cn
lab head
Qijun Chen
contact affiliation	Shenyang Agricultural University
contact email	qijunchen759@syau.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD014133

PRIDE project URI

• PRIDE
  1. PXD014133
     1. Label: PRIDE project
     2. Name: Global lysine crotonylation and 2-hydroxyisobutyrylation in phenotypically different Toxoplasma gondii parasites