PXD014126

PXD014126 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Quantitative LC-MSMS of human muscle cells after electrical pulse stimulation
Description	We hypothesized that muscle contraction produces a cellular stress signal capable to increase lipolysis to sustain fuel availability during exercise. The aim of the present study was to identify novel exercise-regulated myokines, aka exerkines, able to promote lipolysis in human adipocytes. To this end, human primary myotubes from lean healthy volunteers were submitted to electrical pulse stimulation to mimic either acute intense or chronic moderate exercise. Conditioned media experiments with hMADS adipocytes were performed. Unbiased proteomic and ELISA analyses were applied in conditioned media and human plasma samples. Real-time qPCR was performed in cultured myotubes and muscle biopsy samples.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:05:22.259.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fabrice BERTILE
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-04 08:59:01	ID requested
1	2020-05-26 14:16:34	announced
⏵ 2	2024-10-22 05:05:30	announced	2024-10-22: Updated project metadata.

Publication List

10.1172/jci.insight.131870;
Laurens C, Parmar A, Murphy E, Carper D, Lair B, Maes P, Vion J, Boulet N, Fontaine C, Marqu, è, s M, Larrouy D, Harant I, Thalamas C, Montastier E, Caspar-Bauguil S, Bourlier V, Tavernier G, Grolleau JL, Bouloumi, é A, Langin D, Viguerie N, Bertile F, Blanc S, de Glisezinski I, O'Gorman D, Moro C, Growth and differentiation factor 15 is secreted by skeletal muscle during exercise and promotes lipolysis in humans. JCI Insight, 5(6):(2020) [pubmed]

10.1172/jci.insight.131870;

Laurens C, Parmar A, Murphy E, Carper D, Lair B, Maes P, Vion J, Boulet N, Fontaine C, Marqu, è, s M, Larrouy D, Harant I, Thalamas C, Montastier E, Caspar-Bauguil S, Bourlier V, Tavernier G, Grolleau JL, Bouloumi, é A, Langin D, Viguerie N, Bertile F, Blanc S, de Glisezinski I, O'Gorman D, Moro C, Growth and differentiation factor 15 is secreted by skeletal muscle during exercise and promotes lipolysis in humans. JCI Insight, 5(6):(2020) [pubmed]

Keyword List

submitter keyword: Human myotubes, LC-MSMS, lipolysis, myokines, exercise, adipose tissue, secretome

submitter keyword: Human myotubes, LC-MSMS, lipolysis, myokines, exercise, adipose tissue, secretome

Contact List

Sarah Cianférani
contact affiliation	Laboratoire de Spectrométrie de Masse BioOrganique, Analytical Science department, IPHC, CNRS, Strasbourg University, UMR7178, Strasbourg, France
contact email	sarah.cianferani@unistra.fr
lab head
Fabrice BERTILE
contact affiliation	CNRS
contact email	fbertile@unistra.fr
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD014126

PRIDE project URI

Repository Record List

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