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PXD014126

PXD014126 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative LC-MSMS of human muscle cells after electrical pulse stimulation
DescriptionWe hypothesized that muscle contraction produces a cellular stress signal capable to increase lipolysis to sustain fuel availability during exercise. The aim of the present study was to identify novel exercise-regulated myokines, aka exerkines, able to promote lipolysis in human adipocytes. To this end, human primary myotubes from lean healthy volunteers were submitted to electrical pulse stimulation to mimic either acute intense or chronic moderate exercise. Conditioned media experiments with hMADS adipocytes were performed. Unbiased proteomic and ELISA analyses were applied in conditioned media and human plasma samples. Real-time qPCR was performed in cultured myotubes and muscle biopsy samples.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:05:22.259.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFabrice BERTILE
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-06-04 08:59:01ID requested
12020-05-26 14:16:34announced
22024-10-22 05:05:30announced2024-10-22: Updated project metadata.
Publication List
10.1172/jci.insight.131870;
Laurens C, Parmar A, Murphy E, Carper D, Lair B, Maes P, Vion J, Boulet N, Fontaine C, Marqu, è, s M, Larrouy D, Harant I, Thalamas C, Montastier E, Caspar-Bauguil S, Bourlier V, Tavernier G, Grolleau JL, Bouloumi, é A, Langin D, Viguerie N, Bertile F, Blanc S, de Glisezinski I, O'Gorman D, Moro C, Growth and differentiation factor 15 is secreted by skeletal muscle during exercise and promotes lipolysis in humans. JCI Insight, 5(6):(2020) [pubmed]
Keyword List
submitter keyword: Human myotubes, LC-MSMS, lipolysis, myokines, exercise, adipose tissue, secretome
Contact List
Sarah Cianférani
contact affiliationLaboratoire de Spectrométrie de Masse BioOrganique, Analytical Science department, IPHC, CNRS, Strasbourg University, UMR7178, Strasbourg, France
contact emailsarah.cianferani@unistra.fr
lab head
Fabrice BERTILE
contact affiliationCNRS
contact emailfbertile@unistra.fr
dataset submitter
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Dataset FTP location
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PRIDE project URI
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