PXD014120

PXD014120 is an original dataset announced via ProteomeXchange.

Title	Proteomic and metabolomic analysis of Azospirillum brasilense ntrC mutant under high and low nitrogen conditions
Description	Azospirillum brasilense is a free-living diazothophic bacterium that associates with important grasses and cereals, promoting plant growth and increasing crop productivity. The nitrogen metabolism in this organism is tightly regulated by the availability of nitrogen and by the Ntr regulatory system. This system comprises the GlnD protein, which is capable of adding uridylyl groups to the PII proteins, GlnB and GlnZ, under limiting nitrogen levels. Under such conditions, the histidine kinase NtrB cannot interact with GlnB and phosphorylate NtrC. When phosphorylated, NtrC is a transcriptional activator of genes involved in metabolism of alternative nitrogen sources. Considering the key role of NtrC in nitrogen metabolism in Azospirillum brasilense, in this work we evaluated the proteomic and metabolomic profiles of the wild type FP2 strain and its mutant ntrC grown under high and low nitrogen. Analysis of the integrated data identifies, novel NtrC targets, including proteins involved in the response against oxidative stressnm (i.e., glutathione S-transferase and hydroperoxide resistance protein,), underlining the importance of NtrC to bacterial survival under oxidative stress conditions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:56:34.635.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gustavo De Souza
SpeciesList	scientific name: Azospirillum brasilense FP2; NCBI TaxID: 1262469;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-04 06:12:27	ID requested
1	2019-10-14 01:40:05	announced
⏵ 2	2024-10-22 04:56:35	announced	2024-10-22: Updated project metadata.

Kukolj C, Pedrosa FO, de Souza GA, Sumner LW, Lei Z, Sumner B, do Amaral FP, Juexin W, Trupti J, Huergo LF, Monteiro RA, Valdameri G, Stacey G, de Souza EM, Mutant under High and Low Nitrogen Conditions. J Proteome Res, 19(1):92-105(2020) [pubmed]

10.1021/acs.jproteome.9b00397;

submitter keyword: Azospirillum brasilense. NtrC. Nitrogen metabolism. Proteomic. Metabolomic. Setaria viridis

Emmanuel M Souza
contact affiliation	Dept. of Biochemistry, Universidade Federal do Parana, Curitiba-PR, Brazil
contact email	souzaem@ufpr.br
lab head
Gustavo De Souza
contact affiliation	Dept. of Biochemistry, Federal University of Rio Grande do Norte
contact email	gadsouza@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014120
     1. Label: PRIDE project
     2. Name: Proteomic and metabolomic analysis of Azospirillum brasilense ntrC mutant under high and low nitrogen conditions