PXD014111

PXD014111 is an original dataset announced via ProteomeXchange.

Title	iTRAQ-based quantitative proteomic analysis of nuclear fractions of glioma (WHO grade II, III and IV) tissues to identify altered transcriptional regulatory proteins
Description	Glioma tumors arise from normal glial cells (astrocytes). Gliomas are morphologically and clinically classified into four malignancy grades as Grade I, II, III and IV. Grade I and II comprise low grade gliomas that grow slowly, are well differentiated and have a better prognosis and survival compared to grades III and IV. In contrast, grade III-IV lack endothelial proliferation and are highly vascular with a tendency to infiltrate and have areas of extensive necrosis and hypoxia. In the present study, we used iTRAQ (isobaric tags for relative and absolute quantitation) - based quantitative proteomic approach followed by online liquid chromatography and high resolution tandem mass spectrometry (LC-MS/MS) to identify differential nuclear proteins enriched with transcriptional regulatory function from three different grades of astrocytoma tumors (WHO Grade II, III and IV) compared to control.
HostingRepository	PRIDE
AnnounceDate	2019-08-06
AnnouncementXML	Submission_2019-08-06_02:46:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ravi Sirdeshmukh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; methylthiolated residue; monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-04 01:39:53	ID requested
⏵ 1	2019-08-06 02:46:53	announced

Gupta MK, Polisetty RV, Sharma R, Ganesh RA, Gowda H, Purohit AK, Ankathi P, Prasad K, Mariswamappa K, Lakshmikantha A, Uppin MS, Sundaram C, Gautam P, Sirdeshmukh R, Altered transcriptional regulatory proteins in glioblastoma and YBX1 as a potential regulator of tumor invasion. Sci Rep, 9(1):10986(2019) [pubmed]

submitter keyword: Glioma, diffused astrocytoma, anaplastic astrocytoma, Glioblastoma, sub cellular fractionation, nuclear proteins, iTRAQ, transcription factor, transcription regulatory proteins

Dr. Ravi Sirdeshmukh
contact affiliation	Institute of Bioinformatics, Bangalore - 560066, India
contact email	ravisirdeshmukh@gmail.com
lab head
Ravi Sirdeshmukh
contact affiliation	Institute of Bioinformatics
contact email	ravisirdeshmukh@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD014111

PRIDE project URI

• PRIDE
  1. PXD014111
     1. Label: PRIDE project
     2. Name: iTRAQ-based quantitative proteomic analysis of nuclear fractions of glioma (WHO grade II, III and IV) tissues to identify altered transcriptional regulatory proteins