PXD014098 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | CLUH-dependent RNA granules coordinate mitochondrial catabolic pathways with mTORC1 signaling and mitophagy |
Description | Mitochondria house anabolic and catabolic processes that must be balanced and rapidly adjusted to meet the cellular demands. CLUH binds mRNAs of nuclear encoded mitochondrial proteins and is highly expressed in the liver, where it regulates metabolic plasticity by an ill-defined mechanism. Here, we show that CLUH-dependent regulation of mitochondrial function is spatially organized through the coalescence of CLUH and its target mRNAs in specific G3BP1-positive granules. These CLUH-dependent granules protect mRNAs involved in mitochondrial energy-converting pathways from decay and define their translational fate to match nutrient availability. CLUH granules suppress premature mTORC1 activation during nutrient deprivation, inhibiting mitochondrial anabolic pathways. Lack of spatial CLUH regulation causes mTORC1 hyperactivation, which impairs mitophagy and triggers cell death. Our data demonstrate that the temporal catabolic adaptation of mitochondria depends on a compartmentalized CLUH-dependent post-transcriptional mechanism that controls mTORC1 signaling and mitochondrial turnover, thus ensuring survival. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:00:58.743.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Janica Wiederstein |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-06-03 06:09:06 | ID requested | |
1 | 2020-03-19 05:14:07 | announced | |
⏵ 2 | 2024-10-22 05:00:59 | announced | 2024-10-22: Updated project metadata. |
Publication List
Pla-Mart, í, n D, Schatton D, Wiederstein JL, Marx MC, Khiati S, Kr, ü, ger M, Rugarli EI, CLUH granules coordinate translation of mitochondrial proteins with mTORC1 signaling and mitophagy. EMBO J, 39(9):e102731(2020) [pubmed] |
10.15252/embj.2019102731; |
Keyword List
curator keyword: Biological |
submitter keyword: Mitochondria, catabolism, RNA-binding proteins |
Contact List
Elena Irene Rugarli |
contact affiliation | Institute for Genetics, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 50931 Cologne, Germany |
contact email | elena.rugarli@uni-koeln.de |
lab head | |
Janica Wiederstein |
contact affiliation | Institute for Genetics/CECAD (AG Krüger) |
contact email | jwieders@uni-koeln.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014098
- Label: PRIDE project
- Name: CLUH-dependent RNA granules coordinate mitochondrial catabolic pathways with mTORC1 signaling and mitophagy