PXD014096

PXD014096 is an original dataset announced via ProteomeXchange.

Title	Label-free proteomic approach to study the non-lethal effects of silver nanoparticles on a biofilm gut model
Description	Among all the food-related nanoparticles consumed every day, silver nanoparticles (AgNPs),due totheir anti-microbial properties, are one of the most commonly utilized. Despite this, the effects of sub-lethal concentrations of AgNPs, especially on gut biofilms, has been poorly investigated. To address these issues, we investigated the proteomic response of a mono-species Escherichia coli gut biofilm, used as in-vitro human gut model, to chronic and acute exposure of sub-lethal concentrations(1 µg/mL) of AgNPs. We used a new gel and label-free proteomic approach based on shotgun nanoflow scale liquid chromatography-tandem mass spectrometry (LC-MS/MS) that, respect to the traditional proteomic investigation, allows a higher dynamic range of quantification of the whole proteome. To assess all different possible exposure scenarios, we compared the total proteome of four samples: untreated cells, cells treated with AgNPs for 24h (acute treatment), for 96h (chronic treatment), and cells grown in the presence of AgNPs for 96h and treated again for 24h (acute and chronic treatment). Proteomic profiling provided new insight into the mechanisms exerted by AgNPs highlighting the important role of the bacterial biofilm in protecting intestinal epithelial cells. Among the 1917 proteins identified, 216 were ANOVA significant and several pathways resulted altered including biofilm formation, bacterial adhesion, ROS stress response and glucose utilization.
HostingRepository	PRIDE
AnnounceDate	2019-10-31
AnnouncementXML	Submission_2019-10-31_03:12:14.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guido Domingo
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-03 05:17:08	ID requested
⏵ 1	2019-10-31 03:12:15	announced

Dataset with its publication pending

submitter keyword: label free proteomics, silver nanoparticles, non-lethal effects, gut biofilm, acute and chronic exposure

Dr.ssa Milena Marsoni
contact affiliation	Lab. Fisiologia vegetale. Uninsubria, Varese . Italy
contact email	milena.marsoni@gmail.com
lab head
Guido Domingo
contact affiliation	Uninsubria
contact email	guidodomingo.biologo@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014096
     1. Label: PRIDE project
     2. Name: Label-free proteomic approach to study the non-lethal effects of silver nanoparticles on a biofilm gut model