PXD014094

PXD014094 is an original dataset announced via ProteomeXchange.

Title	Identification of Cavin3 interacting proteins
Description	Caveolae are specialized domains of the plasma membrane. Formation of these invaginations is dependent on the expression of Caveolin-1 or -3 and proteins of the cavin family. In response to stress, caveolae disassemble and cavins are released from caveolae, allowing cavins to potentially interact with intracellular targets. Here, we describe the intracellular (non-plasma membrane) cavin interactome using biotin affinity proteomics and mass spectrometry. We validate 47 potential cavin-interactor proteins using a cell-free expression system and protein-protein binding assays. These data, together with pathway analyses, revealed novel roles for cavin proteins in metabolism and stress signaling. We validated the interaction between one candidate interactor protein, protein phosphatase 1 alpha (PP1α), and Cavin-1 and -3 and show that UV treatment causes release of Cavin3 from caveolae allowing interaction with, and inhibition of, PP1α. This interaction increases H2AX phosphorylation to stimulate apoptosis, identifying a pro-apoptotic signaling pathway from surface caveolae to the nucleus.
HostingRepository	PRIDE
AnnounceDate	2019-07-23
AnnouncementXML	Submission_2019-08-13_05:27:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kerrie-Ann McMahon
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-03 04:11:38	ID requested
1	2019-07-23 08:57:12	announced
⏵ 2	2019-08-13 05:27:49	announced	Updated publication reference for PubMed record(s): 31332168.

McMahon KA, Wu Y, Gambin Y, Sierecki E, Tillu VA, Hall T, Martel N, Okano S, Moradi SV, Ruelcke JE, Ferguson C, Yap AS, Alexandrov K, Hill MM, Parton RG, Identification of intracellular cavin target proteins reveals cavin-PP1alpha interactions regulate apoptosis. Nat Commun, 10(1):3279(2019) [pubmed]

ProteomeXchange project tag: Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: caveolae, cavins

Robert Parton
contact affiliation	The University of Queensland, Institute for Molecular Bioscience, Division of Cell Biology and Molecular Medicine, Building 80, Services Rd, St Lucia, Brisbane, Queensland 4072, Australia
contact email	r.parton@imb.uq.edu.au
lab head
Kerrie-Ann McMahon
contact affiliation	University of Queensland, Institute for Molecular Bioscience
contact email	k.mcmahon3@uq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD014094

PRIDE project URI

• PRIDE
  1. PXD014094
     1. Label: PRIDE project
     2. Name: Identification of Cavin3 interacting proteins