PXD014092

PXD014092 is an original dataset announced via ProteomeXchange.

Title	The RNA fold interactome of evolutionary conserved yeast RNA structures in S. cerevisiae
Description	Based on previous DMS-Seq data that provides in vivo structural conformation of RNA, we here applied a streamlined RNA pull-down approach to study 186 evolutionary conserved RNA folds in S. cerevisiae. These folds bound 162 different proteins, with the majority known or inferred to be RNA-binding proteins. In contrast to global RNA interactome capture methods; we were able to associate individual messenger RNAs harboring conserved RNA folds with their set of bound regulating proteins. Our data suggests a more widespread function of tRNA synthetases as mRNA regulators. Using an in vivo RNA reporter screen, we show that specific mRNA-RBP pairs regulate protein expression, which connects structural RNA features recognized by the RNA-binding protein to functionality.
HostingRepository	PRIDE
AnnounceDate	2020-07-02
AnnouncementXML	Submission_2020-07-01_23:18:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	F Butter
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-03 03:41:08	ID requested
1	2020-07-01 23:15:37	announced
⏵ 2	2020-07-01 23:18:36	announced	2020-07-02: Updated publication reference for PubMed record(s): 32313017, 32493961.

Casas-Vila N, Sayols S, P, é, rez-Mart, í, nez L, Scheibe M, Butter F, The RNA fold interactome of evolutionary conserved RNA structures in S. cerevisiae. Nat Commun, 11(1):2789(2020) [pubmed]

Weng ML, Chen WK, Chen XY, Lu H, Sun ZR, Yu Q, Sun PF, Xu YJ, Zhu MM, Jiang N, Zhang J, Zhang JP, Song YL, Ma D, Zhang XP, Miao CH, pathway suppression. Nat Commun, 11(1):1869(2020) [pubmed]

curator keyword: Biological

submitter keyword: yeast

RBP

interactomics

Falk Butter
contact affiliation	Institute of Molecular Biology (IMB) Ackermannweg 4, 55128 Mainz, Germany
contact email	f.butter@imb.de
lab head
F Butter
contact affiliation	Quantitative Proteomics Institute of Molecular Biology (IMB)
contact email	f.butter@imb-mainz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD014092

PRIDE project URI

• PRIDE
  1. PXD014092
     1. Label: PRIDE project
     2. Name: The RNA fold interactome of evolutionary conserved yeast RNA structures in S. cerevisiae