PXD014090

PXD014090 is an original dataset announced via ProteomeXchange.

Title	New Insights into Heat Responses in Grape Leaves through Combined Phosphoproteomic and Acetylproteomic Analyses
Description	Heat stress is a serious and widespread threat to the quality and yield of many crops, including grape (Vitis vinifera), which is cultivated worldwide. Here, we conducted phosphoproteomic and acetylproteomic analyses of leaves from grape cultivated in four distinct temperature regimes. The phosphorylation or acetylation of a total of 1,011 phosphoproteins with 1,828 phosphosites, and 96 acetylproteins with 148 acetylsites, changed when plants were grown at 35°C, 40°C and 45°C in comparison with the proteome profiles of plants grown at 25°C. The greatest number of changes were observed at higher temperatures. Functional classification and enrichment analysis indicated that phosphorylation, rather than acetylation, of serine/arginine-rich splicing factors was involved in the responses to high temperatures. This is congruent with previous observations that alternative splicing events occurred more frequently in grapevine under high temperature. Changes in acetylation patterns were more common than changes in phosphorylation patterns in photosynthesis-related proteins at high temperatures, while heat shock proteins were more associated with modifications in phosphorylation than in acetylation. Nineteen proteins were identified with changes in both phosphorylation and acetylation, consistent with crosstalk between these post-translational modifications.
HostingRepository	PRIDE
AnnounceDate	2019-11-13
AnnouncementXML	Submission_2019-11-12_19:21:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guotian Liu
SpeciesList	scientific name: Vitis vinifera (Grape); NCBI TaxID: 29760;
ModificationList	phosphorylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-31 08:38:37	ID requested
⏵ 1	2019-11-12 19:21:29	announced

Liu GT, Jiang JF, Liu XN, Jiang JZ, Sun L, Duan W, Li RM, Wang Y, Lecourieux D, Liu CH, Li SH, Wang LJ, New insights into the heat responses of grape leaves via combined phosphoproteomic and acetylproteomic analyses. Hortic Res, 6():100(2019) [pubmed]

submitter keyword: grape, heat,phosphoproteome,acetylproteome

Li-Jun Wang
contact affiliation	Beijing Key Laboratory of Grape Science and Enology and Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
contact email	ljwang@ibcas.ac.cn
lab head
Guotian Liu
contact affiliation	Northwest A&F University
contact email	liuguotian555@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014090
     1. Label: PRIDE project
     2. Name: New Insights into Heat Responses in Grape Leaves through Combined Phosphoproteomic and Acetylproteomic Analyses