PXD014084

PXD014084 is an original dataset announced via ProteomeXchange.

Title	XAB2 involved in RNA processing.
Description	RNA splicing and the DNA damage response are intriguingly linked in mammals but the underlying mechanisms remain poorly understood. Using an in vivo biotinylation tagging approach in mice we show that XAB2, the human homologue of the yeast pre-mRNA-splicing factor SYF1 has a functional role in Nucleotide Excision Repair (NER) and the DNA damage response (DDR) in mammals. XAB2 interacts with spliceosome factors and is part of the core spliceosome that binds to spliceosomal U4 and U6 snRNAs during hepatic development. Ablation of XAB2 leads to defective NER, intron retention, the aberrant accumulation of pre-mRNAs and to a faulty ATM/ATR DDR signaling. Using functional approaches, we find that XAB2 dissociates from RNA targets upon persistent DNA damage or transcription blockage and from spliceosomal RNAs in the NER-defective developing livers. Thus, XAB2 functionally links NER to the spliceosomal response to DNA damage during hepatic development with important ramifications for transcription-coupled DNA repair disorders.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:26:00.801.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jeroen Demmers
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-31 03:24:25	ID requested
1	2021-09-08 09:43:01	announced
⏵ 2	2024-10-22 05:26:08	announced	2024-10-22: Updated project metadata.

Goulielmaki E, Tsekrekou M, Batsiotos N, Ascens, ã, o-Ferreira M, Ledaki E, Stratigi K, Chatzinikolaou G, Topalis P, Kosteas T, Altm, ü, ller J, Demmers JA, Barbosa-Morais NL, Garinis GA, The splicing factor XAB2 interacts with ERCC1-XPF and XPG for R-loop processing. Nat Commun, 12(1):3153(2021) [pubmed]

10.1038/s41467-021-23505-1;

submitter keyword: snRNAs,XAB2, RNA processing, in vivo biotinylation, interaction analysis, pre-mRNAs, nucleotide excision repair

Jeroen Demmers
contact affiliation	Proteomics Center, Erasmus MC, Rotterdam, the Netherlands
contact email	j.demmers@erasmusmc.nl
lab head
Jeroen Demmers
contact affiliation	Proteomics Center, Erasmus University Medical Center, Rotterdam, The Netherlands
contact email	j.demmers@erasmusmc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD014084

PRIDE project URI

• PRIDE
  1. PXD014084
     1. Label: PRIDE project
     2. Name: XAB2 involved in RNA processing.