PXD014077

PXD014077 is an original dataset announced via ProteomeXchange.

Title	Proteomic dissection of CFAP44 deficient axonemes
Description	Leishmania are protists (class: Kinetoplastida) with a single multifunctional flagellum, which forms a canonical motile 9+2 microtubule axoneme in the promastigote forms. Deletion of gene CFAP44 (LmxM.14.1430) caused a reduction in promastigote motility (Beneke et al., PLoS Pathogens, 2019; accepted). To study the changes in protein composition in CFAP44 deficient axonemes, flagellar skeletons were isolated from CFAP44 knockout mutants and the parental cell line L. mex Cas9 T7 (Beneke et al., R Soc Open Sci. 2017; 4(5):170095) using a modified version of the method by Robinson et al., (Methods Enzymol. 1991; 196:285-99). Liquid chromatography tandem mass spectrometry and a label-free quantitation method (SINQ; Trudgian et al., 2011, Proteomics 10.1002) were used to identify proteins enriched in each fraction. This PRIDE upload contains .RAW and .XML files, as well as the SINQ quantification output file “SINQ_raw_data”. XML files are named SUB9810; MSS11680. .RAW files are structured as follows: “CFAP44 mutants: Qex01_SVH_180422_TomBeneke_B29_F_001”; “Cas9 T7 parentals: Qex01_SVH_180422_TomBeneke_Cas9_F_005”.
HostingRepository	PRIDE
AnnounceDate	2020-01-21
AnnouncementXML	Submission_2020-01-21_00:02:36.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tom Beneke
SpeciesList	scientific name: Leishmania mexicana MHOM/GT/2001/U1103; NCBI TaxID: 929439;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-30 11:02:45	ID requested
⏵ 1	2020-01-21 00:02:37	announced

Beneke T, Banecki K, Fochler S, Gluenz E, flagella. J Cell Sci, 133(2):(2020) [pubmed]

submitter keyword: Leishmania, flagellum, CFAP44, axoneme

Dr Eva Gluenz
contact affiliation	Royal Society University Research Fellow and Group Leader Research Lecturer, Sir William Dunn School of Pathology, University of Oxford, Fellow by special election, St. Edmund Hall, University of Oxford
contact email	eva.gluenz@path.ox.ac.uk
lab head
Tom Beneke
contact affiliation	University of Oxford, Sir William Dunn School of Pathology
contact email	tom.beneke@path.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD014077

PRIDE project URI

• PRIDE
  1. PXD014077
     1. Label: PRIDE project
     2. Name: Proteomic dissection of CFAP44 deficient axonemes