PXD014077 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic dissection of CFAP44 deficient axonemes |
Description | Leishmania are protists (class: Kinetoplastida) with a single multifunctional flagellum, which forms a canonical motile 9+2 microtubule axoneme in the promastigote forms. Deletion of gene CFAP44 (LmxM.14.1430) caused a reduction in promastigote motility (Beneke et al., PLoS Pathogens, 2019; accepted). To study the changes in protein composition in CFAP44 deficient axonemes, flagellar skeletons were isolated from CFAP44 knockout mutants and the parental cell line L. mex Cas9 T7 (Beneke et al., R Soc Open Sci. 2017; 4(5):170095) using a modified version of the method by Robinson et al., (Methods Enzymol. 1991; 196:285-99). Liquid chromatography tandem mass spectrometry and a label-free quantitation method (SINQ; Trudgian et al., 2011, Proteomics 10.1002) were used to identify proteins enriched in each fraction. This PRIDE upload contains .RAW and .XML files, as well as the SINQ quantification output file “SINQ_raw_data”. XML files are named SUB9810; MSS11680. .RAW files are structured as follows: “CFAP44 mutants: Qex01_SVH_180422_TomBeneke_B29_F_001”; “Cas9 T7 parentals: Qex01_SVH_180422_TomBeneke_Cas9_F_005”. |
HostingRepository | PRIDE |
AnnounceDate | 2020-01-21 |
AnnouncementXML | Submission_2020-01-21_00:02:36.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tom Beneke |
SpeciesList | scientific name: Leishmania mexicana MHOM/GT/2001/U1103; NCBI TaxID: 929439; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-05-30 11:02:45 | ID requested | |
⏵ 1 | 2020-01-21 00:02:37 | announced | |
Publication List
Beneke T, Banecki K, Fochler S, Gluenz E, flagella. J Cell Sci, 133(2):(2020) [pubmed] |
Keyword List
submitter keyword: Leishmania, flagellum, CFAP44, axoneme |
Contact List
Dr Eva Gluenz |
contact affiliation | Royal Society University Research Fellow and Group Leader Research Lecturer, Sir William Dunn School of Pathology, University of Oxford, Fellow by special election, St. Edmund Hall, University of Oxford |
contact email | eva.gluenz@path.ox.ac.uk |
lab head | |
Tom Beneke |
contact affiliation | University of Oxford, Sir William Dunn School of Pathology |
contact email | tom.beneke@path.ox.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014077
- Label: PRIDE project
- Name: Proteomic dissection of CFAP44 deficient axonemes