PXD014076

PXD014076 is an original dataset announced via ProteomeXchange.

Title	The phosphoproteome of the coral model system Aiptasia
Description	The endosymbiotic interaction established by cnidarians and photosynthetic dinoflagellate algae is the foundation of coral reef ecosystems. This essential interaction is globally threatened to breakdown by anthropogenic disturbance. As such, it is compelling to understand the molecular mechanisms underpinning the cnidarian-algal association. We investigated phosphorylation-mediated protein signaling as a mechanism of regulation of the cnidarian-algal interaction, and we report on the generation of the first phosphoproteome for the coral model system Aiptasia. Using mass spectrometry-based phosphoproteomics in data-independent acquisition (DIA) allowed consistent quantification of over 3,000 phosphopeptides totaling more than 1,600 phosphoproteins across aposymbiotic (symbiont-free) and symbiotic anemones. Additionally, to allow for discrimination between translational regulation and post-translational phosphorylation, we generated a total proteome dataset from the same anemones and used it for phosphopeptide normalization against protein amount. While quantification of protein phosphorylation relied upon the generation of a spectrum library generated by data-dependent acquisition (DDA), total protein quantification in DIA was conducted "library-free" (directDIA) in SpectronautX. DirectDIA allowed consistent quantification of 20,215 peptides, totaling 4,121 proteins (3,518 protein groups) across biological samples.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:56:07.859.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fabia Simona
SpeciesList	scientific name: Exaiptasia pallida; NCBI TaxID: 1720309;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-30 11:01:56	ID requested
1	2019-11-06 05:43:58	announced
2	2019-11-12 03:31:13	announced	2019-11-12: Updated publication reference for PubMed record(s): 31693769.
3	2020-01-10 07:57:12	announced	2020-01-10: Updated project metadata.
⏵ 4	2024-10-22 04:56:11	announced	2024-10-22: Updated project metadata.

Simona F, Zhang H, Voolstra CR, Evidence for a role of protein phosphorylation in the maintenance of the cnidarian-algal symbiosis. Mol Ecol, 28(24):5373-5386(2019) [pubmed]

10.1111/mec.15298;

submitter keyword: Aiptasia,Cnidarian, Coral-model, spectral library, DIA/SWATH-MS, DDA, quantification

Christian Robert Voolstra
contact affiliation	Professor of Genetics of Adaptation in Aquatic Systems Department of Biology University of Konstanz Germany
contact email	christian.voolstra@uni-konstanz.de
lab head
Fabia Simona
contact affiliation	King Abdullah University of Science and Technology (KAUST)
contact email	fabia.simona@kaust.edu.sa
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD014076

PRIDE project URI

• PRIDE
  1. PXD014076
     1. Label: PRIDE project
     2. Name: The phosphoproteome of the coral model system Aiptasia