PXD014052

PXD014052 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomics of a stenotopic caddisfly (Trichoptera) identifies acclimation strategies
Description	As a result of increasing thermal fluctuations and mean temperature values, organisms will experience conditions beyond their physiological limits. In situ adaptation to thermal regimes is mediated via directional selection and phenotypic plasticity. The latter involves physiological and morphological adjustments realized by underlying molecular mechanisms. Understanding species' adaptive capacities requires investigating these adjustive processes. Yet, acclimation through phenotypic plasticity remains largely unexplored, especially at the molecular level; For example, whether cold-adapted species inhabiting freshwater spring ecosystems have evolved adaptive mechanisms to cope with warming of freshwater habitats has, to our knowledge, never been investigated. This work reports a comprehensive proteomics study of the stenotopic species Crunoecia irrorata (Curtis, 1834) (Trichoptera: Lepistomatidae) acclimated to 10, 15 and 20 °C for 168 h. A liquid chromatography tandem mass spectrometry (LC-MS/MS)-based shotgun proteomics approach identified molecular mechanisms underlying acclimation. We constructed a homology-based database by combining genomic and transcriptomic data from related species and quantified 1356 proteins, of which 186 were differentially expressed between temperature treatments. Through functional annotation, we identified candidate proteins facilitating, among others, trehalose accumulation, tracheal system alteration, and heat shock protein regulation, then discuss concomitant ecophysiological implications. These results provide new insights into the mechanisms of adaptive responses to warming of species inhabiting freshwater ecosystems sensitive to climate change. Further, identified candidate proteins will aid in developing targeted experiments to understand their compensatory physiology. To our knowledge, this is the first study utilizing this approach to investigate the nature of phenotypic plasticity of aquatic macroinvertebrates.
HostingRepository	PRIDE
AnnounceDate	2019-10-10
AnnouncementXML	Submission_2019-10-10_08:43:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joshua Ebner
SpeciesList	scientific name: Crunoecia irrorata; NCBI TaxID: 1876254;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-28 23:26:39	ID requested
1	2019-08-08 07:56:17	announced
⏵ 2	2019-10-10 08:43:11	announced	2019-10-10: Updated project metadata.

Ebner JN, Ritz D, von Fumetti S, Comparative proteomics of stenotopic caddisfly Crunoecia irrorata identifies acclimation strategies to warming. Mol Ecol, 28(19):4453-4469(2019) [pubmed]

curator keyword: Biological

submitter keyword: Environmental proteomics, acclimation, molecular adaptation, phenotypic plasticity, freshwater springs, climate change

Joshua Niklas Ebner
contact affiliation	University of Basel, Department of Environmental Sciences, Geoecology Research Group, Klingelbergstrasse 27, 4056 Basel, Switzerland
contact email	joshua.ebner@unibas.ch
lab head
Joshua Ebner
contact affiliation	University of Basel
contact email	joshua.ebner@unibas.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014052
     1. Label: PRIDE project
     2. Name: Comparative proteomics of a stenotopic caddisfly (Trichoptera) identifies acclimation strategies