PXD014043 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Temporal quantitative proteomics of mGluR-induced protein translation and phosphorylation in neurons |
Description | At neuronal synapses, activation of group I metabotropic glutamate receptors (mGluR1/5) triggers a form of long-term depression (mGluR-LTD) that relies on new protein synthesis and the internalization of AMPA-type glutamate receptors. Dysregulation of these processes has been implicated in the development of mental disorders such as autism spectrum disorders and therefore merit a better understanding on a molecular level. Here, to study mGluR-induced signaling pathways, we integrated quantitative phosphoproteomics with the analyses of newly synthesized proteins via bio-orthogonal amino acids (azidohomoalanine) in a pulsed labeling strategy in cultured hippocampal neurons stimulated with DHPG, a specific agonist for group I mGluRs. We identified several kinases with important roles in DHPG-induced mGluR activation, which we confirmed using small molecule kinase inhibitors. Furthermore, changes in the AMPA receptor endocytosis pathway in both protein synthesis and protein phosphorylation were identified, whereby Intersectin-1 was validated as a novel player in this pathway. This study revealed several new insights into the molecular pathways downstream of group I mGluR activation in hippocampal neurons, and provides a rich resource for further analyses. |
HostingRepository | PRIDE |
AnnounceDate | 2020-09-11 |
AnnouncementXML | Submission_2020-09-15_23:10:52.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Charlotte van Gelder |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-05-28 05:27:37 | ID requested | |
1 | 2020-09-11 04:35:36 | announced | |
⏵ 2 | 2020-09-15 23:13:30 | announced | 2020-09-16: Updated publication reference for PubMed record(s): 32912969. |
Publication List
van Gelder CAGH, Penning R, Veth TS, Catsburg LAE, Hoogenraad CC, MacGillavry HD, Altelaar M, Temporal Quantitative Proteomics of mGluR-induced Protein Translation and Phosphorylation in Neurons. Mol Cell Proteomics, 19(12):1952-1968(2020) [pubmed] |
Keyword List
submitter keyword: mGluR-LTD, proteomics, phosphoproteomics, synaptic plasticity, protein translation, AMPA receptor, bioorthogonal amino acid tagging, metabolic labeling, tandem mass tags |
Contact List
Maarten Altelaar |
contact affiliation | 1 Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands, 2 Netherlands Proteomics Center, Padualaan 8, 3584 CH Utrecht, The Netherlands |
contact email | m.altelaar@uu.nl |
lab head | |
Charlotte van Gelder |
contact affiliation | Utrecht University |
contact email | c.a.g.h.vangelder@uu.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD014043 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014043
- Label: PRIDE project
- Name: Temporal quantitative proteomics of mGluR-induced protein translation and phosphorylation in neurons