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PXD014030

PXD014030 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChIP-MS of GR and in mouse liver
DescriptionThe Glucocorticoid Receptor (GR) is a potent metabolic regulator and a major drug target. While GR was shown to play various important roles in circadian biology, its rhythmic genomic actions have never been studied. Here we mapped GR’s genome-wide chromatin occupancy in mouse livers throughout the day/night cycle. We show how GR partitions metabolic processes during fasting (cellular maintenance, gluconeogenesis) and feeding (lipid and amino acid metabolism) by time-dependent binding and target gene regulation. Highlighting the dominant role GR plays in synchronizing circadian pathways, we find that the majority of oscillating genes harbor GR binding sites and depend on GR for amplitude stability . Surprisingly, this rhythmic pattern is altered by exposure to high fat diet in a ligand-independent manner. We show how the remodeling of oscillatory gene expression and GR binding result from a concomitant increase with Stat5 co-occupancy in obese mice, and that loss of GR reduces circulating glucose and triglycerides differentially during feeding and fasting. Altogether, our findings highlight GR’s fundamental role in the rhythmic orchestration of hepatic metabolism.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:55:00.600.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMichael Wierer
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-05-28 01:44:43ID requested
12019-10-24 02:17:05announced
22024-10-22 04:55:01announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: coregulator, hormone, steroid receptor,chromatin
Contact List
Matthias Mann
contact affiliationDepartment of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Munich, Germany
contact emailmmann@biochem.mpg.de
lab head
Michael Wierer
contact affiliationDepartment of Proteomics and Signal Transduction, Max-Planck Institute of Biochemistry, Martinsried, Germany
contact emailwierer@biochem.mpg.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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