PXD014022

PXD014022 is an original dataset announced via ProteomeXchange.

Title	The Response of A549 Cells to Aspergillus fumigatus and Pseudomonas aeruginosa: A Proteomic perspective
Description	Aspergillus fumigatus and Pseudomonas aeruginosa are the most prevalent fungal and bacterial pathogens, associated with cystic fibrosis-related infections, respectively. Although it is well established that P. aeruginosa eventually predominates as the primary pathogen, it is unknown why this is the case. Label-free quantitative proteomics was employed here to compare the cellular response to A. fumigatus, P. aeruginosa and sequential exposure to both pathogens in the type II alveolar epithelial cell line, A549, a model of the alveolar surface. In total, 2264 proteins were identified and the analysis of statistically significant (p<0.05; ±1.5 fold change), differentially abundant (SSDA) proteins, revealed an increase in the relative abundance of proteins associated with biological processes common to all pathogen-exposed groups. These included mitochondrial stress, energy metabolism and protein processing in the endoplasmic reticulum. Conversely, clear differences were observed between the cellular response to A. fumigatus or P. aeruginosa and following sequential exposure to both pathogens. The relative abundance of proteins associated with ribosomal activity appeared comparable between sequentially exposed cells and unexposed cells, but were substantially decreased in fungal- and bacterial-exposed cells. Furthermore, compared to unexposed cells, the relative abundance of proteins associated with the actin cytoskeleton, endocytosis, phagosome and lysosome were increased in A. fumigatus and P. aeruginosa-exposed cells, but appeared unchanged or decreased in sequentially exposed cells. This research provides novel insights into the whole cell proteomic response to A. fumigatus and P. aeruginosa, and highlights distinct differences following sequential exposure to both pathogens.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:56:47.135.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Carolan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-27 07:02:09	ID requested
1	2019-11-11 02:58:07	announced
⏵ 2	2024-10-22 04:56:55	announced	2024-10-22: Updated project metadata.

Margalit A, Kavanagh K, Carolan JC, . J Proteome Res, 19(1):279-291(2020) [pubmed]

10.1021/acs.jproteome.9b00520;

submitter keyword: LFQ MS, A549 Cell Line,Aspergillus fumigatus, Pseudomonas aeruginosa, Cystic Fibrosis

James Carolan
contact affiliation	Department of Biology, Maynooth University, Maynooth, Co Kildare, Ireland.
contact email	james.carolan@nuim.ie
lab head
James Carolan
contact affiliation	Biology
contact email	james.carolan@nuim.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014022
     1. Label: PRIDE project
     2. Name: The Response of A549 Cells to Aspergillus fumigatus and Pseudomonas aeruginosa: A Proteomic perspective