PXD014016

PXD014016 is an original dataset announced via ProteomeXchange.

Title	Complexome profiling of heart mitochondria from mouse expressing alternative oxidase
Description	Ciona intestinalis alternative oxidase (AOX) is a mitochondrial respiratory enzyme that acts as an alternative electron sink for ubiquinol (reduced quinone) when the cytochrome bc1 complex (complex III) and/or cytochrome c oxidase (complex IV) are inhibited or impaired (El-Khoury et al., 2014). AOX thus enables electron flux through the mitochondrial electron transport chain (ETC) and thereby prevents reverse electron transport from succinate dehydrogenase (complex II) to NADH:ubiquinone oxidoreductase (complex I) and mitochondrial ROS production, restores redox balance and Krebs cycle activity. As a non-proton-motive enzyme, however, AOX does not directly support ATP production. Previously, a mouse model was generated that despite ubiquitous expression of AOX revealed no deviations from normal physiology and which now is used as model to test mitochondrial disease paradigms (Szibor et al., 2017).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:56:41.004.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilka Wittig
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-27 00:28:35	ID requested
1	2019-12-13 00:36:01	announced
⏵ 2	2024-10-22 04:56:41	announced	2024-10-22: Updated project metadata.

10.1016/j.bbabio.2019.148137;

Szibor M, Gainutdinov T, Fernandez-Vizarra E, Dufour E, Gizatullina Z, Debska-Vielhaber G, Heidler J, Wittig I, Viscomi C, Gellerich F, Moore AL, Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: Switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg, 1861(2):148137(2020) [pubmed]

curator keyword: Biological

submitter keyword: alternative oxidase, mitochondria,LC-MSMS, complexome profiling

Marten Szibor
contact affiliation	BioMediTech & Tampere University Hospital, Faculty of Medicine and Health Technology, Tampere University, Arvo Ylpön katu 34 (ARVO), FI-33520 Tampere, Finland
contact email	marten.szibor@tuni.fi
lab head
Ilka Wittig
contact affiliation	Functional Proteomics, Goethe University, Frankfurt am Main , Germany
contact email	wittig@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD014016

PRIDE project URI

• PRIDE
  1. PXD014016
     1. Label: PRIDE project
     2. Name: Complexome profiling of heart mitochondria from mouse expressing alternative oxidase