PXD014009

PXD014009 is an original dataset announced via ProteomeXchange.

Title	Lamin A molecular compression and sliding as mechanisms behind nucleoskeleton elasticity (complementary data of PXD008337)
Description	Lamin A is a nuclear intermediate filament protein critical for nuclear architecture and mechanics and mutated in a wide range of human diseases. Yet little is known about the molecular architecture of lamins and mechanisms of their assembly. Here we use cross-linking SILAC mass spectrometry to determine interactions within lamin dimers and between dimers in higher-order polymers. We find evidence for a compression mechanism where coiled coils in the lamin A rod can slide onto each other to contract rod length, likely driven by a wide range of electrostatic interactions with the flexible linkers between coiled coils. Similar interactions occur with unstructured regions flanking the rod domain during oligomeric assembly. Mutations linked to human disease block these interactions, suggesting that this spring-like contraction can explain in part the dynamic mechanical stretch and flexibility properties of the lamin polymer and other intermediate filament networks.
HostingRepository	PRIDE
AnnounceDate	2019-07-12
AnnouncementXML	Submission_2019-07-16_02:02:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Zou
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-24 06:58:05	ID requested
1	2019-07-12 03:39:23	announced
⏵ 2	2019-07-16 02:02:08	announced	Updated publication reference for PubMed record(s): 31296869.

Makarov AA, Zou J, Houston DR, Spanos C, Solovyova AS, Cardenal-Peralta C, Rappsilber J, Schirmer EC, Lamin A molecular compression and sliding as mechanisms behind nucleoskeleton elasticity. Nat Commun, 10(1):3056(2019) [pubmed]

submitter keyword: lamin A, coiled coil, disordered linker, compression

Juri Rappsilber
contact affiliation	Room 4.18a Michael Swann Building Max Born Crescent Edinburgh EH9 3BF UK
contact email	juri.rappsilber@ed.ac.uk
lab head
Juan Zou
contact affiliation	The University of Edinburgh
contact email	jzou@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD014009

PRIDE project URI

• PRIDE
  1. PXD014009
     1. Label: PRIDE project
     2. Name: Lamin A molecular compression and sliding as mechanisms behind nucleoskeleton elasticity (complementary data of PXD008337)