PXD014008

PXD014008 is an original dataset announced via ProteomeXchange.

Title	A MYC2, MYC3, MYC4-dependent transcription factor network regulates touch responses in plants
Description	Plants are continuously exposed to environmental triggers, including mechanical stimulation. Our results demonstrate that jasmonic acid (JA)-signalling plays a key role in very early gene expression changes, well before it leads to touch-induced developmental changes. We show that the JA-activated transcription factors MYC2/MYC3/MYC4 co-regulate touch-induced gene expression of 266 genes, many of which peak in induction around 25 minutes and then rapidly decline by 40-60 minutes. ChIP-seq shows that MYC2 dynamically binds hundreds of touch-induced promoters within 25 minutes. Promoter activation assays confirm that MYC2 directly activates these touch-induced promoters. By combining multi-omic data, we have identified a core MYC2/3/4-dependent ‘touch regulon’, containing many previously-unknown MYC2 targets like bHLH19 and ERF109. We show bHLH19 can in turn directly activate the ORA47 promoter, indicating that MYC2/3/4 initiate a hierarchical network of downstream transcription factors. Through hormone profiling we reveal the rapid touch-induced accumulation of JA/JA-isoleucine is directly controlled by MYC2/3/4 in a positive amplification loop regulating JA-biosynthesis genes.
HostingRepository	PRIDE
AnnounceDate	2019-10-29
AnnouncementXML	Submission_2019-11-06_00:30:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Owen Duncan
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-24 06:55:49	ID requested
1	2019-10-29 03:44:04	announced
⏵ 2	2019-11-06 00:30:41	announced	2019-11-06: Updated publication reference for PubMed record(s): 31662474.

Van Moerkercke A, Duncan O, Zander M, Š, imura J, Broda M, Vanden Bossche R, Lewsey MG, Lama S, Singh KB, Ljung K, Ecker JR, Goossens A, Millar AH, Van Aken O, A MYC2/MYC3/MYC4-dependent transcription factor network regulates water spray-responsive gene expression and jasmonate levels. Proc Natl Acad Sci U S A, 116(46):23345-23356(2019) [pubmed]

submitter keyword: MYC2, touch respose, arabidopsis

Olivier Van Aken
contact affiliation	Lund University Sölvegatan 35 223 62 – Lund Sweden
contact email	olivier.van_aken@biol.lu.se
lab head
Owen Duncan
contact affiliation	UWA
contact email	owen.duncan@uwa.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD014008

PRIDE project URI

• PRIDE
  1. PXD014008
     1. Label: PRIDE project
     2. Name: A MYC2, MYC3, MYC4-dependent transcription factor network regulates touch responses in plants