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PXD014008 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA MYC2, MYC3, MYC4-dependent transcription factor network regulates touch responses in plants
DescriptionPlants are continuously exposed to environmental triggers, including mechanical stimulation. Our results demonstrate that jasmonic acid (JA)-signalling plays a key role in very early gene expression changes, well before it leads to touch-induced developmental changes. We show that the JA-activated transcription factors MYC2/MYC3/MYC4 co-regulate touch-induced gene expression of 266 genes, many of which peak in induction around 25 minutes and then rapidly decline by 40-60 minutes. ChIP-seq shows that MYC2 dynamically binds hundreds of touch-induced promoters within 25 minutes. Promoter activation assays confirm that MYC2 directly activates these touch-induced promoters. By combining multi-omic data, we have identified a core MYC2/3/4-dependent ‘touch regulon’, containing many previously-unknown MYC2 targets like bHLH19 and ERF109. We show bHLH19 can in turn directly activate the ORA47 promoter, indicating that MYC2/3/4 initiate a hierarchical network of downstream transcription factors. Through hormone profiling we reveal the rapid touch-induced accumulation of JA/JA-isoleucine is directly controlled by MYC2/3/4 in a positive amplification loop regulating JA-biosynthesis genes.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterOwen Duncan
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-05-24 06:55:49ID requested
12019-10-29 03:44:04announced
22019-11-06 00:30:41announced2019-11-06: Updated publication reference for PubMed record(s): 31662474.
Publication List
Van Moerkercke A, Duncan O, Zander M, Šimura J, Broda M, Vanden Bossche R, Lewsey MG, Lama S, Singh KB, Ljung K, Ecker JR, Goossens A, Millar AH, Van Aken O, A MYC2/MYC3/MYC4-dependent transcription factor network regulates water spray-responsive gene expression and jasmonate levels. Proc Natl Acad Sci U S A, 116(46):23345-23356(2019) [pubmed]
Keyword List
submitter keyword: MYC2, touch respose, arabidopsis
Contact List
Olivier Van Aken
contact affiliationLund University Sölvegatan 35 223 62 – Lund Sweden
contact emailolivier.van_aken@biol.lu.se
lab head
Owen Duncan
contact affiliationUWA
contact emailowen.duncan@uwa.edu.au
dataset submitter
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